Anti-inflammatory effects of the prostacyclin analogue iloprost in an in vitro model of inflamed human dental pulp cells

Rahman Wahyudi; Sonntana Seang; Vincent Everts; Thanaphum Osathanon; Chalida Nakalekha Limjeerajarus

doi:10.1111/aej.12736

Anti-inflammatory effects of the prostacyclin analogue iloprost in an in vitro model of inflamed human dental pulp cells

Rahman Wahyudi, Sonntana Seang, Vincent Everts, Thanaphum Osathanon, Chalida Nakalekha Limjeerajarus^*

^*Corresponding author for this work

Oral Cell Biology

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Iloprost's anti-inflammatory effects on human dental pulp stem cells (HDPCs) are currently unknown. We hypothesized that iloprost could downregulate the expression of inflammatory-related genes and protein in an inflamed HDPC in vitro model. To induce inflammation, the HDPCs were treated with a cocktail of interleukin-1 beta, interferon-gamma, and tumour necrosis alpha, at a ratio of 1:10:100. Iloprost (10⁻⁶ M) was then added or not to the cultures. Interleukin-6 (IL-6) and interleukin-12 (IL-12) mRNA expression were assessed by real-time polymerase chain reaction. IL-6 protein expression was assessed by enzyme-linked immunosorbent assay. The results were analysed using one-way ANOVA or the Kruskal–Wallis test. The cytokine cocktail induced more robust IL-6 expression than LPS treatment. Iloprost slightly, yet significantly, downregulated IL-6 and IL-12 mRNA expression. These findings suggest that iloprost might be used as a beneficial component in vital pulp therapy.

Original language	English
Pages (from-to)	330-338
Number of pages	9
Journal	Australian Endodontic Journal
Volume	49
Issue number	S1
Early online date	1 Feb 2023
DOIs	https://doi.org/10.1111/aej.12736
Publication status	Published - Sept 2023

Bibliographical note

Funding Information:
This project was funded by the National Research Council of Thailand (NRCT) RSA63001‐13, Faculty of Dentistry Chulalongkorn Dental Research Grant DRF62001. T.O. is supported by the National Research Council of Thailand (N41A640135). The authors are grateful to Assist. Prof. Dr. Soranun Chantarangsu for the statistical analysis methodology review, Dr. Kevin Tompkins for critical review and language revision of the manuscript, and Prof. Dr. Prasit Pavasant for his guidance and wisdom.

Publisher Copyright:
© 2023 Australian Society of Endodontology Inc.

Funding

This project was funded by the National Research Council of Thailand (NRCT) RSA63001‐13, Faculty of Dentistry Chulalongkorn Dental Research Grant DRF62001. T.O. is supported by the National Research Council of Thailand (N41A640135). The authors are grateful to Assist. Prof. Dr. Soranun Chantarangsu for the statistical analysis methodology review, Dr. Kevin Tompkins for critical review and language revision of the manuscript, and Prof. Dr. Prasit Pavasant for his guidance and wisdom.

Keywords

cytokines
dental pulp
iloprost
in vitro
inflammation

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title = "Anti-inflammatory effects of the prostacyclin analogue iloprost in an in vitro model of inflamed human dental pulp cells",

abstract = "Iloprost's anti-inflammatory effects on human dental pulp stem cells (HDPCs) are currently unknown. We hypothesized that iloprost could downregulate the expression of inflammatory-related genes and protein in an inflamed HDPC in vitro model. To induce inflammation, the HDPCs were treated with a cocktail of interleukin-1 beta, interferon-gamma, and tumour necrosis alpha, at a ratio of 1:10:100. Iloprost (10−6 M) was then added or not to the cultures. Interleukin-6 (IL-6) and interleukin-12 (IL-12) mRNA expression were assessed by real-time polymerase chain reaction. IL-6 protein expression was assessed by enzyme-linked immunosorbent assay. The results were analysed using one-way ANOVA or the Kruskal–Wallis test. The cytokine cocktail induced more robust IL-6 expression than LPS treatment. Iloprost slightly, yet significantly, downregulated IL-6 and IL-12 mRNA expression. These findings suggest that iloprost might be used as a beneficial component in vital pulp therapy.",

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note = "Funding Information: This project was funded by the National Research Council of Thailand (NRCT) RSA63001‐13, Faculty of Dentistry Chulalongkorn Dental Research Grant DRF62001. T.O. is supported by the National Research Council of Thailand (N41A640135). The authors are grateful to Assist. Prof. Dr. Soranun Chantarangsu for the statistical analysis methodology review, Dr. Kevin Tompkins for critical review and language revision of the manuscript, and Prof. Dr. Prasit Pavasant for his guidance and wisdom. Publisher Copyright: {\textcopyright} 2023 Australian Society of Endodontology Inc.",

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AU - Limjeerajarus, Chalida Nakalekha

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N2 - Iloprost's anti-inflammatory effects on human dental pulp stem cells (HDPCs) are currently unknown. We hypothesized that iloprost could downregulate the expression of inflammatory-related genes and protein in an inflamed HDPC in vitro model. To induce inflammation, the HDPCs were treated with a cocktail of interleukin-1 beta, interferon-gamma, and tumour necrosis alpha, at a ratio of 1:10:100. Iloprost (10−6 M) was then added or not to the cultures. Interleukin-6 (IL-6) and interleukin-12 (IL-12) mRNA expression were assessed by real-time polymerase chain reaction. IL-6 protein expression was assessed by enzyme-linked immunosorbent assay. The results were analysed using one-way ANOVA or the Kruskal–Wallis test. The cytokine cocktail induced more robust IL-6 expression than LPS treatment. Iloprost slightly, yet significantly, downregulated IL-6 and IL-12 mRNA expression. These findings suggest that iloprost might be used as a beneficial component in vital pulp therapy.

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Anti-inflammatory effects of the prostacyclin analogue iloprost in an in vitro model of inflamed human dental pulp cells

Abstract

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