Antibacterial and cell penetrating effects of LFcin17-30, LFampin265-284, and LF chimera on enteroaggregative Escherichia coli

R. Reyes-Cortes, E. Acosta-Smith, R. Mondragón-Flores, K. Nazmi, J.G.M. Bolscher, A. Canizalez-Roman, N. Leon-Sicairos

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Lactoferrin (LF) is a protein with antimicrobial activity, which is conferred in part by 2 regions contained in its N-terminal lobe. These regions have been used to develop the following synthetic peptides: lactoferricin17-30, lactoferrampin265-284, and LF chimera (a fusion of lactoferricin17-30 and lactoferrampin265-284). We have reported that these LF peptides have antibacterial activity against several pathogenic bacteria; however, the exact mechanism of action has not been established. Here, we report the effects of LF peptides on the viability of enteroaggregative Escherichia coli (EAEC) and the ability of these peptides to penetrate into the bacteria cytoplasm. The viability of EAEC treated with LF peptides was determined via enumeration of colony-forming units, and the binding and internalization of the LF peptides was followed via immunogold labeling and electron microscopy. Treatment of EAEC with 20 and 40 μmol/L LF peptides reduced bacterial growth compared with untreated bacteria. Initially the peptides associated with the plasma membrane, but after 5 to 30 min of incubation, the peptides were found in the cytoplasm. Remarkably, bacteria treated with LF chimera developed cytosolic electron-dense structures that contained the antimicrobial peptide. Our results suggest that the antibacterial mechanism of LF peptides on EAEC involves their interaction with and penetration into the bacteria.

Original languageEnglish
Pages (from-to)76-81
Number of pages6
JournalBiochemistry and Cell Biology
Volume95
Issue number1
Early online date13 Nov 2016
DOIs
Publication statusPublished - Feb 2017

Bibliographical note

This note is part of a Special Issue from the XIIth Lactoferrin Conference.

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