Antibacterial and osteogenic functionalization of titanium with silicon/copper-doped high-energy shot peening-assisted micro-arc oxidation technique

X. Shen, W. Hu, L. Ping, C. Liu, L. Yao, Z. Deng, G. Wu

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Antibacterial and osteogenic functionalization of titanium (Ti) implants will greatly expand their clinical indications in immediate implant therapy, accelerate osteointegration, and enhance long-term prognosis. We had recently shown that the high-energy shot peening (HESP)-assisted micro-arc oxidation (MAO) significantly improved the bioactivity and coating stability of Ti-based substrates. In this study, we further functionalized Ti with antibacterial and osteogenic properties by doping silicon (Si) and/or copper (Cu) ions into HESP/MAO-treated coatings. Physicochemical characterization displayed that the doping of Si and Cu in HESP/MAO-treated coatings (Si/Cu-MAO) did not significantly change their surface topography, roughness, crystal structure, coating thickness, bonding strength, and wettability. The results of X-ray photoelectron spectroscopy (XPS) showed that Si and Cu in the Si/Cu-MAO coating was in the form of silicate radical (SiO32–) and bivalent copper (Cu2+), respectively. The total amounts of Si and Cu were about 13.5 and 5.8 μg/cm2, which released about 33.2 and 31.3% within 14 day, respectively. Compared with the control group (MAO), Si doping samples (MAO-Si) significantly increased the cell viability, alkaline phosphatase (ALP) activity, mineralization and osteogenic genes (ALP, collagen I and osteocalcin) expression of MC3T3-E1 cells. Furthermore, the addition of Cu presented good bactericidal property against both Staphylococcus aureus and Streptococcus mutans (even under the co-culture condition of bacteria and MC3T3-E1 cells): the bacteriostatic rate of both bacteria was over 95%. In conclusion, the novel bioactive Si/Cu-MAO coating with antibacterial and osteogenic properties is a promising functionalization method for orthopedic and dental implants, especially in the immediate implant treatment with an infected socket.

Original languageEnglish
Article number573464
Number of pages13
JournalFrontiers in Bioengineering and Biotechnology
Volume8
DOIs
Publication statusPublished - 8 Oct 2020

Funding

This work was funded by the National Natural Science Foundation of China (81701016, 81870810, and 31700827), Scientific Research Fund of Zhejiang Provincial Education Department (Y201533871), Zhejiang Provincial Science and Technology Project for Public Welfare (No. LGF20H140002), Zhejiang Provincial Medical and Health Science and Technology Project (2020KY657) and Wenzhou Public Welfare Science and Technology Project (Y20160142 and Y20190099), Wenzhou Medical University Basic Scientific Research Operating Expenses (KYYW201905), and All the authors want to thank Kendrick HiiRuYie for his help of the language embellishment. Funding. This work was funded by the National Natural Science Foundation of China (81701016, 81870810, and 31700827), Scientific Research Fund of Zhejiang Provincial Education Department (Y201533871), Zhejiang Provincial Science and Technology Project for Public Welfare (No. LGF20H140002), Zhejiang Provincial Medical and Health Science and Technology Project (2020KY657) and Wenzhou Public Welfare Science and Technology Project (Y20160142 and Y20190099), Wenzhou Medical University Basic Scientific Research Operating Expenses (KYYW201905), and Open project of State Key Laboratory of Oral Diseases (SKLOD2020OF04).

FundersFunder number
Scientific Research Fund of Zhejiang Provincial Education Department
Wenzhou Public Welfare Science and Technology ProjectY20160142, Y20190099
Zhejiang Provincial Medical and Health Science and Technology Project
Zhejiang Provincial Science and Technology Project for Public Welfare
Wenzhou Medical UniversityKYYW201905
National Natural Science Foundation of China31700827, 81701016, 81870810
Zhejiang Province Public Welfare Technology Application Research ProjectLGF20H140002
State Key Laboratory of Oral DiseaseSKLOD2020OF04
Scientific Research Fund of Liaoning Provincial Education DepartmentY201533871
Medical Science and Technology Project of Zhejiang Province2020KY657

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