Antibacterial CATH-2 Peptide Coating to Prevent Bone Implant-Related Infection

Pardis Keikhosravani, Fatemeh Jahanmard, Tim Bollen, Kamran Nazmi, Edwin J.A. Veldhuizen, Prasad Gonugunta, Prasaanth Ravi Anusuyadevi, Bart C.H. van der Wal, Charles Vogely, Floris J. Bikker, Peyman Taheri, Harrie Weinans, Saber Amin Yavari*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The development of antibacterial coatings is a promising approach to preventing biofilm formation and reducing the overuse of systemic antibiotics. However, widespread antibiotic use has resulted in antibiotic-resistant bacteria, limiting the efficacy of antibiotic-based coatings. Herein, an antibacterial coating is developed by layer-by-layer (LbL) assembly of two polymers namely PDLG (poly (D,L-lactide-co-glycolide)) and gelatin methacryloyl (GelMA) while chicken cathelicidin-2 (CATH-2), a cationic and amphipathic peptide, is loaded between these polymer layers. The electrospray method is used to apply the coatings to achieve efficient peptide loading and durability. The CATH-2 bactericidal concentration ranges are first identified, followed by a study of their cytotoxicity to human mesenchymal stem cells (hMSCs) and macrophage cell lines. Later, different LbL electrospray coating assemblies loaded with the optimal peptide concentration are sought. Various coating strategies are investigated to identify an LbL coating that exhibits prolonged and biocompatible CATH-2 release. The resulting CATH-2-coated titanium surfaces exhibit strong antibacterial activity against both Staphylococcus aureus and Escherichia coli bacteria for 4 days and are biocompatible with hMSCs and macrophage cells. This coating can be considered as a versatile delivery system platform for the delivery of CATH-2 peptides while avoiding cytotoxicity, particularly for the prevention of infections associated with implants.

Original languageEnglish
Article number2300500
Pages (from-to)1-13
Number of pages13
JournalAdvanced Materials Technologies
Volume8
Issue number18
Early online date2 Aug 2023
DOIs
Publication statusPublished - 25 Sept 2023

Bibliographical note

Funding Information:
This publication is part of the project DARTBAC (with project number NWA.1292.19.354) of the research program NWA-ORC which is (partly) financed by the Dutch Research Council (NWO). The kind support of Dr. Azin Khodaei, Dr. Alireza Hassani, Leonardo Cecotto and Nada Rahmani in running the experiment is appreciated.

Funding Information:
This publication is part of the project DARTBAC (with project number NWA.1292.19.354) of the research program NWA‐ORC which is (partly) financed by the Dutch Research Council (NWO). The kind support of Dr. Azin Khodaei, Dr. Alireza Hassani, Leonardo Cecotto and Nada Rahmani in running the experiment is appreciated.

Publisher Copyright:
© 2023 The Authors. Advanced Materials Technologies published by Wiley-VCH GmbH.

Keywords

  • antibacterial peptides
  • antibiotic resistance
  • biomaterial-related infection
  • durable coatings

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