Apoprotein heterogeneity increases spectral disorder and a step-wise modification of the B850 fluorescence peak position

Cristian Ilioaia; Tjaart P.J. Krüger; Oana Ilioaia; Bruno Robert; Rienk van Grondelle; Andrew Gall

doi:10.1016/j.bbabio.2017.11.003

Apoprotein heterogeneity increases spectral disorder and a step-wise modification of the B850 fluorescence peak position

Cristian Ilioaia^*, Tjaart P.J. Krüger, Oana Ilioaia, Bruno Robert, Rienk van Grondelle, Andrew Gall

^*Corresponding author for this work

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Abstract

It has already been established that the quaternary structure of the main light-harvesting complex (LH2) from the photosynthetic bacterium Rhodopseudomonas palustris is a nonameric ‘ring’ of PucAB heterodimers and under low-light culturing conditions an increased diversity of PucB synthesis occurs. In this work, single molecule fluorescence emission studies show that different classes of LH2 ‘rings’ are present in “low-light” adapted cells and that an unknown chaperon process creates multiple sub-types of ‘rings’ with more conformational sub-states and configurations. This increase in spectral disorder significantly augments the cross-section for photon absorption and subsequent energy flow to the reaction centre trap when photon availability is a limiting factor. This work highlights yet another variant used by phototrophs to gather energy for cellular development.

Original language	English
Pages (from-to)	137-144
Number of pages	8
Journal	Biochimica et Biophysica Acta - Bioenergetics
Volume	1859
Issue number	2
Early online date	21 Nov 2017
DOIs	https://doi.org/10.1016/j.bbabio.2017.11.003
Publication status	Published - Feb 2018

Funding

This work was supported by the French Infrastructure for Integrated Structural Biology (FRISBI) ANR-10-INSB-05 (A.G, C.I., B.R); the European Research Council (ERC) through an Advanced Investigator Grant, contract no. 267333 (R.v.G., B.R.); the French Agence Nationale de la Recherché (ANR) through a Chaire d'excellence, ANR-07-CEX-009-01 (A.G., O.I.); the European Union (EU) FP6 MEST-CT-2004-008048 Marie Curie Early Stage Training Network via the Advanced Training in Laser Sciences project (T.P.J.K.); the EU Access to Research Infrastructures action of the Improving Human Potential Program , contract no. RII-CT-2003-506350 (A.G.); CEA-Eurotalents program, European Union contract no. PCOFUND-GA-2008-228664 (C.I.); TOP grant ( 700.58.305 ) from the Foundation of Chemical Sciences, part of the Netherlands Organization for Scientific Research (C.I. and R.v.G.). Appendix A

Funders	Funder number
ANR-07-CEX-009-01
Foundation of Chemical Sciences
Netherlands Organization for Scientific Research
Technology Opportunities Program
European Commission	PCOFUND-GA-2008-228664, 700.58.305, FP6 MEST-CT-2004-008048
European Research Council	267333
Agence Nationale de la Recherche
French Infrastructure for Integrated Structural Biology	ANR-10-INSB-05

Keywords

Light harvesting complexes
Photosynthesis
Purple bacteria
Single molecule spectroscopy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbabio.2017.11.003

Apoprotein heterogeneity increases spectral disorder and a stepwise modification of the B850 fluorescence peak positionFinal published version, 1.51 MBLicence: Article 25fa Dutch Copyright Act

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abstract = "It has already been established that the quaternary structure of the main light-harvesting complex (LH2) from the photosynthetic bacterium Rhodopseudomonas palustris is a nonameric {\textquoteleft}ring{\textquoteright} of PucAB heterodimers and under low-light culturing conditions an increased diversity of PucB synthesis occurs. In this work, single molecule fluorescence emission studies show that different classes of LH2 {\textquoteleft}rings{\textquoteright} are present in “low-light” adapted cells and that an unknown chaperon process creates multiple sub-types of {\textquoteleft}rings{\textquoteright} with more conformational sub-states and configurations. This increase in spectral disorder significantly augments the cross-section for photon absorption and subsequent energy flow to the reaction centre trap when photon availability is a limiting factor. This work highlights yet another variant used by phototrophs to gather energy for cellular development.",

keywords = "Light harvesting complexes, Photosynthesis, Purple bacteria, Single molecule spectroscopy",

author = "Cristian Ilioaia and Kr{\"u}ger, {Tjaart P.J.} and Oana Ilioaia and Bruno Robert and {van Grondelle}, Rienk and Andrew Gall",

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AU - Ilioaia, Cristian

AU - Krüger, Tjaart P.J.

AU - Ilioaia, Oana

AU - Robert, Bruno

AU - van Grondelle, Rienk

AU - Gall, Andrew

PY - 2018/2

Y1 - 2018/2

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AB - It has already been established that the quaternary structure of the main light-harvesting complex (LH2) from the photosynthetic bacterium Rhodopseudomonas palustris is a nonameric ‘ring’ of PucAB heterodimers and under low-light culturing conditions an increased diversity of PucB synthesis occurs. In this work, single molecule fluorescence emission studies show that different classes of LH2 ‘rings’ are present in “low-light” adapted cells and that an unknown chaperon process creates multiple sub-types of ‘rings’ with more conformational sub-states and configurations. This increase in spectral disorder significantly augments the cross-section for photon absorption and subsequent energy flow to the reaction centre trap when photon availability is a limiting factor. This work highlights yet another variant used by phototrophs to gather energy for cellular development.

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KW - Photosynthesis

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Apoprotein heterogeneity increases spectral disorder and a step-wise modification of the B850 fluorescence peak position

Abstract

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Keywords

UN SDGs

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