Abstract
It has already been established that the quaternary structure of the main light-harvesting complex (LH2) from the photosynthetic bacterium Rhodopseudomonas palustris is a nonameric ‘ring’ of PucAB heterodimers and under low-light culturing conditions an increased diversity of PucB synthesis occurs. In this work, single molecule fluorescence emission studies show that different classes of LH2 ‘rings’ are present in “low-light” adapted cells and that an unknown chaperon process creates multiple sub-types of ‘rings’ with more conformational sub-states and configurations. This increase in spectral disorder significantly augments the cross-section for photon absorption and subsequent energy flow to the reaction centre trap when photon availability is a limiting factor. This work highlights yet another variant used by phototrophs to gather energy for cellular development.
| Original language | English |
|---|---|
| Pages (from-to) | 137-144 |
| Number of pages | 8 |
| Journal | Biochimica et Biophysica Acta - Bioenergetics |
| Volume | 1859 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2018 |
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Keywords
- Light harvesting complexes
- Photosynthesis
- Purple bacteria
- Single molecule spectroscopy
Cite this
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Apoprotein heterogeneity increases spectral disorder and a step-wise modification of the B850 fluorescence peak position. / Ilioaia, Cristian; Krüger, Tjaart P.J.; Ilioaia, Oana; Robert, Bruno; van Grondelle, Rienk; Gall, Andrew.
In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1859, No. 2, 02.2018, p. 137-144.Research output: Contribution to Journal › Article › Academic › peer-review
TY - JOUR
T1 - Apoprotein heterogeneity increases spectral disorder and a step-wise modification of the B850 fluorescence peak position
AU - Ilioaia, Cristian
AU - Krüger, Tjaart P.J.
AU - Ilioaia, Oana
AU - Robert, Bruno
AU - van Grondelle, Rienk
AU - Gall, Andrew
PY - 2018/2
Y1 - 2018/2
N2 - It has already been established that the quaternary structure of the main light-harvesting complex (LH2) from the photosynthetic bacterium Rhodopseudomonas palustris is a nonameric ‘ring’ of PucAB heterodimers and under low-light culturing conditions an increased diversity of PucB synthesis occurs. In this work, single molecule fluorescence emission studies show that different classes of LH2 ‘rings’ are present in “low-light” adapted cells and that an unknown chaperon process creates multiple sub-types of ‘rings’ with more conformational sub-states and configurations. This increase in spectral disorder significantly augments the cross-section for photon absorption and subsequent energy flow to the reaction centre trap when photon availability is a limiting factor. This work highlights yet another variant used by phototrophs to gather energy for cellular development.
AB - It has already been established that the quaternary structure of the main light-harvesting complex (LH2) from the photosynthetic bacterium Rhodopseudomonas palustris is a nonameric ‘ring’ of PucAB heterodimers and under low-light culturing conditions an increased diversity of PucB synthesis occurs. In this work, single molecule fluorescence emission studies show that different classes of LH2 ‘rings’ are present in “low-light” adapted cells and that an unknown chaperon process creates multiple sub-types of ‘rings’ with more conformational sub-states and configurations. This increase in spectral disorder significantly augments the cross-section for photon absorption and subsequent energy flow to the reaction centre trap when photon availability is a limiting factor. This work highlights yet another variant used by phototrophs to gather energy for cellular development.
KW - Light harvesting complexes
KW - Photosynthesis
KW - Purple bacteria
KW - Single molecule spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85035761466&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85035761466&partnerID=8YFLogxK
U2 - 10.1016/j.bbabio.2017.11.003
DO - 10.1016/j.bbabio.2017.11.003
M3 - Article
VL - 1859
SP - 137
EP - 144
JO - Biochimica et Biophysica Acta (BBA) - Bioenergetics
JF - Biochimica et Biophysica Acta (BBA) - Bioenergetics
SN - 0005-2728
IS - 2
ER -