Appearance of interleukin-1 in macrophages and in ramified microglia in the brain of endotoxin-treated rats: a pathway for the induction of non-specific symptoms of sickness?

A M van Dam, M Brouns, S Louisse, F Berkenbosch

    Research output: Contribution to JournalArticleAcademicpeer-review

    Abstract

    The presence and cellular localization of interleukin-1 beta immunoreactivity (irIL-1) in and around the brain was investigated using immunocytochemistry on Bouin's fixed vibratome brain sections of control and endotoxin-treated rats. Peripheral administration of endotoxin resulted in the appearance of irIL-1 in cells in the meninges, choroid plexus, brain blood vessels and in non-neuronal cells in the brain parenchyma. Using monoclonal and polyclonal antibodies to macrophage and astrocyte antigens, the endotoxin-induced irIL-1 positive cells could be identified as macrophages in the meninges and choroid plexus (ED2), perivascular cells (ED2) and ramified microglial cells (GSA-I-B4 isolectin). Our data demonstrate a pathway for the induction of non-specific sickness symptoms in response to endotoxin.

    Original languageEnglish
    Pages (from-to)291-6
    Number of pages6
    JournalBrain Research
    Volume588
    Issue number2
    DOIs
    Publication statusPublished - 21 Aug 1992

    Keywords

    • Animals
    • Brain/cytology
    • Endotoxins/pharmacology
    • Immunohistochemistry
    • Interleukin-1/metabolism
    • Macrophages/metabolism
    • Male
    • Neuroglia/drug effects
    • Rats
    • Rats, Wistar

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