Appearance of interleukin-1 in macrophages and in ramified microglia in the brain of endotoxin-treated rats: a pathway for the induction of non-specific symptoms of sickness?

A M van Dam; M Brouns; S Louisse; F Berkenbosch

doi:10.1016/0006-8993(92)91588-6

Appearance of interleukin-1 in macrophages and in ramified microglia in the brain of endotoxin-treated rats: a pathway for the induction of non-specific symptoms of sickness?

A M van Dam, M Brouns, S Louisse, F Berkenbosch

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

The presence and cellular localization of interleukin-1 beta immunoreactivity (irIL-1) in and around the brain was investigated using immunocytochemistry on Bouin's fixed vibratome brain sections of control and endotoxin-treated rats. Peripheral administration of endotoxin resulted in the appearance of irIL-1 in cells in the meninges, choroid plexus, brain blood vessels and in non-neuronal cells in the brain parenchyma. Using monoclonal and polyclonal antibodies to macrophage and astrocyte antigens, the endotoxin-induced irIL-1 positive cells could be identified as macrophages in the meninges and choroid plexus (ED2), perivascular cells (ED2) and ramified microglial cells (GSA-I-B4 isolectin). Our data demonstrate a pathway for the induction of non-specific sickness symptoms in response to endotoxin.

Original language	English
Pages (from-to)	291-6
Number of pages	6
Journal	Brain Research
Volume	588
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(92)91588-6 https://doi.org/10.1016/0006-8993(92)91588-6
Publication status	Published - 21 Aug 1992

Keywords

Animals
Brain/cytology
Endotoxins/pharmacology
Immunohistochemistry
Interleukin-1/metabolism
Macrophages/metabolism
Male
Neuroglia/drug effects
Rats
Rats, Wistar

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@article{b7c5a24440594c8d89e6c9a62d810728,

title = "Appearance of interleukin-1 in macrophages and in ramified microglia in the brain of endotoxin-treated rats: a pathway for the induction of non-specific symptoms of sickness?",

abstract = "The presence and cellular localization of interleukin-1 beta immunoreactivity (irIL-1) in and around the brain was investigated using immunocytochemistry on Bouin's fixed vibratome brain sections of control and endotoxin-treated rats. Peripheral administration of endotoxin resulted in the appearance of irIL-1 in cells in the meninges, choroid plexus, brain blood vessels and in non-neuronal cells in the brain parenchyma. Using monoclonal and polyclonal antibodies to macrophage and astrocyte antigens, the endotoxin-induced irIL-1 positive cells could be identified as macrophages in the meninges and choroid plexus (ED2), perivascular cells (ED2) and ramified microglial cells (GSA-I-B4 isolectin). Our data demonstrate a pathway for the induction of non-specific sickness symptoms in response to endotoxin.",

keywords = "Animals, Brain/cytology, Endotoxins/pharmacology, Immunohistochemistry, Interleukin-1/metabolism, Macrophages/metabolism, Male, Neuroglia/drug effects, Rats, Rats, Wistar",

author = "{van Dam}, {A M} and M Brouns and S Louisse and F Berkenbosch",

year = "1992",

month = aug,

day = "21",

doi = "10.1016/0006-8993(92)91588-6",

language = "English",

volume = "588",

pages = "291--6",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier B.V.",

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}

van Dam, AM, Brouns, M, Louisse, S & Berkenbosch, F 1992, 'Appearance of interleukin-1 in macrophages and in ramified microglia in the brain of endotoxin-treated rats: a pathway for the induction of non-specific symptoms of sickness?', Brain Research, vol. 588, no. 2, pp. 291-6. https://doi.org/10.1016/0006-8993(92)91588-6, https://doi.org/10.1016/0006-8993(92)91588-6

TY - JOUR

T1 - Appearance of interleukin-1 in macrophages and in ramified microglia in the brain of endotoxin-treated rats

T2 - a pathway for the induction of non-specific symptoms of sickness?

AU - van Dam, A M

AU - Brouns, M

AU - Louisse, S

AU - Berkenbosch, F

PY - 1992/8/21

Y1 - 1992/8/21

N2 - The presence and cellular localization of interleukin-1 beta immunoreactivity (irIL-1) in and around the brain was investigated using immunocytochemistry on Bouin's fixed vibratome brain sections of control and endotoxin-treated rats. Peripheral administration of endotoxin resulted in the appearance of irIL-1 in cells in the meninges, choroid plexus, brain blood vessels and in non-neuronal cells in the brain parenchyma. Using monoclonal and polyclonal antibodies to macrophage and astrocyte antigens, the endotoxin-induced irIL-1 positive cells could be identified as macrophages in the meninges and choroid plexus (ED2), perivascular cells (ED2) and ramified microglial cells (GSA-I-B4 isolectin). Our data demonstrate a pathway for the induction of non-specific sickness symptoms in response to endotoxin.

AB - The presence and cellular localization of interleukin-1 beta immunoreactivity (irIL-1) in and around the brain was investigated using immunocytochemistry on Bouin's fixed vibratome brain sections of control and endotoxin-treated rats. Peripheral administration of endotoxin resulted in the appearance of irIL-1 in cells in the meninges, choroid plexus, brain blood vessels and in non-neuronal cells in the brain parenchyma. Using monoclonal and polyclonal antibodies to macrophage and astrocyte antigens, the endotoxin-induced irIL-1 positive cells could be identified as macrophages in the meninges and choroid plexus (ED2), perivascular cells (ED2) and ramified microglial cells (GSA-I-B4 isolectin). Our data demonstrate a pathway for the induction of non-specific sickness symptoms in response to endotoxin.

KW - Animals

KW - Brain/cytology

KW - Endotoxins/pharmacology

KW - Immunohistochemistry

KW - Interleukin-1/metabolism

KW - Macrophages/metabolism

KW - Male

KW - Neuroglia/drug effects

KW - Rats

KW - Rats, Wistar

U2 - 10.1016/0006-8993(92)91588-6

DO - 10.1016/0006-8993(92)91588-6

M3 - Article

C2 - 1393581

SN - 0006-8993

VL - 588

SP - 291

EP - 296

JO - Brain Research

JF - Brain Research

IS - 2

ER -

Appearance of interleukin-1 in macrophages and in ramified microglia in the brain of endotoxin-treated rats: a pathway for the induction of non-specific symptoms of sickness?

Abstract

Keywords

UN SDGs

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