Abstract
Objective: This study aimed to compare the efficiency of three imidazole-based ionic liquids (ILs) in the microwave-assisted extraction (MAE) of trans-resveratrol from Gnetum gnemon seeds. Methods: Trans-resveratrol was quantitatively extracted using 1-hexyl-3-methylimidazolium bromide ([hmim]Br), 1-butyl-3-methylimidazolium chloride ([bmim]Cl), and 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim]BF4) in a microwave oven. Extraction parameters, including solvent concentration, liquid-solid ratio, and extraction time, were optimized using response surface methodology on the basis of the Box-Behnken design. A reverse-phase high-performance liquid chromatography method was used to determine resveratrol content with acetonitrile: water (75:25 v/v) as the mobile phase. The total phenolic content was determined by the Folin-Ciocalteu method. Results: Under optimal conditions, the resveratrol content when extraction was performed using [hmim]Br, [bmim]Cl, and [bmim]BF4 was 0.068, 0.059, and 0.038 mg/g dry weight, respectively, and total phenolic content was 0.647, 1.736, and 1.436 mg gallic acid equivalent/g, respectively.
Original language | English |
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Pages (from-to) | 39-43 |
Number of pages | 5 |
Journal | International Journal of Applied Pharmaceutics |
Volume | 10 |
Issue number | Special Issue 1 |
DOIs | |
Publication status | Published - 1 Dec 2018 |
Externally published | Yes |
Funding
The authors acknowledge the Directorate of Research and Humanity Engagement, Universitas Indonesia, for financial support through grant “Hibah PITTA 2017.” The authors also thank Prof. Dr. Ibrahim Jantan, M.Sc. of Universiti Kebangsaan, Malaysia, for supervising the work and editing this article.
Funders | Funder number |
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Directorate of Research and Humanity Engagement | |
Universitas Indonesia |
Keywords
- Gnetum gnemon L.
- Ionic liquid
- Melinjo
- Microwave-assisted extraction
- Response surface methodology
- Resveratrol