Application of modular control analysis to inhibition of the adenine nucleotide translocator by palmitoyl-CoA.

J. Ciapaite, G. van Eikenhorst, K. Krab

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Modular kinetic analysis was used to characterize inhibition of adenine nucleotide translocation by palmitoyl-CoA in isolated rat-liver mitochondria. To this purpose, oxidative phosphorylation has been divided into two modules with the fraction of matrix ATP as linking intermediate. The adenine nucleotide translocator is the matrix ATP-consuming module and the remainder of oxidative phosphorylation (ATP synthesis, respiratory chain and transport of phosphates and respiratory substrate) is the matrix ATP-producing module. We found that palmitoyl-CoA inhibits ATP-consuming module (ANT) and has no effect on ATP-producing module. There were no significant differences between kinetic curves obtained with oligomycin and myxothiazol, inhibitors that have opposite effect on membrane potential, suggesting that the use of the fraction of matrix ATP as the only intermediate is a good approximation. A new method has been used to determine the fraction of ATP in the mitochondrial matrix.
Original languageEnglish
Pages (from-to)13-16
JournalMolecular Biology Reports
Volume29
DOIs
Publication statusPublished - 2002

Fingerprint

Dive into the research topics of 'Application of modular control analysis to inhibition of the adenine nucleotide translocator by palmitoyl-CoA.'. Together they form a unique fingerprint.

Cite this