Arrangement of photosystem II supercomplexes in crystalline macrodomains within the thylakoid membranes of green plants

E.J. Boekema, J.F.L. van Breemen, H. van Roon, J.P. Dekker

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The chloroplast thylakoid membrane of green plants is organized in stacked grana membranes and unstacked stroma membranes. We investigated the structural organization of Photosystem II (PSII) in paired grana membrane fragments by transmission electron microscopy. The membrane fragments were obtained by a short treatment of thylakoid membranes with the mild detergent n-dodecyl-α,D-maltoside and are thought to reflect the grana membranes in a native state. The membranes frequently show crystalline macrodomains in which PSII is organized in rows spaced by either 26.3 nm (large-spaced crystals) or 23 nm (small-spaced crystals). The small-spaced crystals are less common but better ordered. Image analysis of the crystals by an aperiodic approach revealed the precise positions of the core parts of PSII in the lattices, as well as features of the peripheral light-harvesting antenna. Together, they indicate that the so-called C
Original languageEnglish
Pages (from-to)1123-1133
JournalJournal of Molecular Biology
Volume301
DOIs
Publication statusPublished - 2000

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