Assay of pemoline in human plasma, saliva and urine by capillary gas chromatography with nitrogen-selective detection

N P Vermeulen, M W Teunissen, D D Breimer

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

A simple gas chromatographic assay of the psycho-stimulant pemoline in human urine, plasma and saliva has been developed. Instead of direct extraction of the drug from urine, plasma and saliva, it is hydrolyzed to 5-phenyl-2,4-oxazolidine-dione with 1 N hydrochloric acid. After extraction this compound is methylated with diazomethane and determined by gas-liquid chromatography using a capillary SCOT column with a mixed stationary phase, a solid injection system and a nitrogen-selective detector. 5-Phenyl-2,4-oxazolidinedione, which was also found to be a metabolite of pemoline, could be determined quantitatively in human urine.

Original languageEnglish
Pages (from-to)133-40
Number of pages8
JournalJournal of Chromatography A
Volume157
DOIs
Publication statusPublished - 21 Sep 1978

Keywords

  • Chromatography, Gas
  • Humans
  • Nitrogen
  • Pemoline
  • Saliva
  • Journal Article

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