Assay of pemoline in human plasma, saliva and urine by capillary gas chromatography with nitrogen-selective detection

N P Vermeulen; M W Teunissen; D D Breimer

doi:10.1016/S0021-9673(00)92329-X

Assay of pemoline in human plasma, saliva and urine by capillary gas chromatography with nitrogen-selective detection

N P Vermeulen, M W Teunissen, D D Breimer

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

A simple gas chromatographic assay of the psycho-stimulant pemoline in human urine, plasma and saliva has been developed. Instead of direct extraction of the drug from urine, plasma and saliva, it is hydrolyzed to 5-phenyl-2,4-oxazolidine-dione with 1 N hydrochloric acid. After extraction this compound is methylated with diazomethane and determined by gas-liquid chromatography using a capillary SCOT column with a mixed stationary phase, a solid injection system and a nitrogen-selective detector. 5-Phenyl-2,4-oxazolidinedione, which was also found to be a metabolite of pemoline, could be determined quantitatively in human urine.

Original language	English
Pages (from-to)	133-40
Number of pages	8
Journal	Journal of Chromatography A
Volume	157
DOIs	https://doi.org/10.1016/S0021-9673(00)92329-X
Publication status	Published - 21 Sept 1978

Keywords

Chromatography, Gas
Humans
Nitrogen
Pemoline
Saliva
Journal Article

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0021-9673(00)92329-X

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title = "Assay of pemoline in human plasma, saliva and urine by capillary gas chromatography with nitrogen-selective detection",

abstract = "A simple gas chromatographic assay of the psycho-stimulant pemoline in human urine, plasma and saliva has been developed. Instead of direct extraction of the drug from urine, plasma and saliva, it is hydrolyzed to 5-phenyl-2,4-oxazolidine-dione with 1 N hydrochloric acid. After extraction this compound is methylated with diazomethane and determined by gas-liquid chromatography using a capillary SCOT column with a mixed stationary phase, a solid injection system and a nitrogen-selective detector. 5-Phenyl-2,4-oxazolidinedione, which was also found to be a metabolite of pemoline, could be determined quantitatively in human urine.",

keywords = "Chromatography, Gas, Humans, Nitrogen, Pemoline, Saliva, Journal Article",

author = "Vermeulen, {N P} and Teunissen, {M W} and Breimer, {D D}",

year = "1978",

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doi = "10.1016/S0021-9673(00)92329-X",

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journal = "Journal of Chromatography A",

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T1 - Assay of pemoline in human plasma, saliva and urine by capillary gas chromatography with nitrogen-selective detection

AU - Vermeulen, N P

AU - Teunissen, M W

AU - Breimer, D D

PY - 1978/9/21

Y1 - 1978/9/21

N2 - A simple gas chromatographic assay of the psycho-stimulant pemoline in human urine, plasma and saliva has been developed. Instead of direct extraction of the drug from urine, plasma and saliva, it is hydrolyzed to 5-phenyl-2,4-oxazolidine-dione with 1 N hydrochloric acid. After extraction this compound is methylated with diazomethane and determined by gas-liquid chromatography using a capillary SCOT column with a mixed stationary phase, a solid injection system and a nitrogen-selective detector. 5-Phenyl-2,4-oxazolidinedione, which was also found to be a metabolite of pemoline, could be determined quantitatively in human urine.

AB - A simple gas chromatographic assay of the psycho-stimulant pemoline in human urine, plasma and saliva has been developed. Instead of direct extraction of the drug from urine, plasma and saliva, it is hydrolyzed to 5-phenyl-2,4-oxazolidine-dione with 1 N hydrochloric acid. After extraction this compound is methylated with diazomethane and determined by gas-liquid chromatography using a capillary SCOT column with a mixed stationary phase, a solid injection system and a nitrogen-selective detector. 5-Phenyl-2,4-oxazolidinedione, which was also found to be a metabolite of pemoline, could be determined quantitatively in human urine.

KW - Chromatography, Gas

KW - Humans

KW - Nitrogen

KW - Pemoline

KW - Saliva

KW - Journal Article

U2 - 10.1016/S0021-9673(00)92329-X

DO - 10.1016/S0021-9673(00)92329-X

M3 - Article

C2 - 701438

SN - 0021-9673

VL - 157

SP - 133

EP - 140

JO - Journal of Chromatography A

JF - Journal of Chromatography A

ER -

Assay of pemoline in human plasma, saliva and urine by capillary gas chromatography with nitrogen-selective detection

Abstract

Keywords

UN SDGs

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