Assembly of the major light-harvesting complex II in lipid nanodiscs

A. Pandit, N Shirzad-Wasei, L.M. Wlodarczyk, H. Van Roon, E.J. Boekema, J.P. Dekker, W.J. de Grip

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Self-aggregation of isolated plant light-harvesting complexes (LHCs) upon detergent extraction is associated with fluorescence quenching and is used as an in vitro model to study the photophysical processes of nonphotochemical quenching (NPQ). In the NPQ state, in vivo induced under excess solar light conditions, harmful excitation energy is safely dissipated as heat. To prevent self-aggregation and probe the conformations of LHCs in a lipid environment devoid from detergent interactions, we assembled LHCII trimer complexes into lipid nanodiscs consisting of a bilayer lipid matrix surrounded by a membrane scaffold protein (MSP). The LHCII nanodiscs were characterized by fluorescence spectroscopy and found to be in an unquenched, fluorescent state. Remarkably, the absorbance spectra of LHCII in lipid nanodiscs show fine structure in the carotenoid and Q
Original languageEnglish
Pages (from-to)2507-2515
JournalBiophysical Journal
Publication statusPublished - 2011


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