Atrophy in non-locomotor muscle in patients with end-stage renal failure

G.K. Sakkas; D. Ball; T.H. Mercer; A.J. Sargeant; K. Tolfrey; P.F. Naish

doi:10.1093/ndt/gfg325

Atrophy in non-locomotor muscle in patients with end-stage renal failure

G.K. Sakkas, D. Ball, T.H. Mercer, A.J. Sargeant, K. Tolfrey, P.F. Naish

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Abstract

Background. All previous histological studies of skeletal muscles of patients with renal failure have used locomotor muscle biopsies. It is thus unclear to what degree the observed abnormalities are due to the uraemic state and how much is due to disuse. The present study was undertaken to attempt to investigate this question by examining a non-locomotor muscle (rectus abdominis) in patients with end-stage renal failure. Methods. Biopsies from rectus abdominis were obtained from 22 renal failure patients (RFPs) undergoing surgical Tenchkoff catheter implantation for peritoneal dialysis and 20 control subjects undergoing elective abdominal surgery. Histochemical staining of frozen sections and morphometric analysis was used to estimate the proportion of each fibre type, muscle fibre area and capillary density. Myosin heavy chain composition was examined by SDS-PAGE. Results. There were no differences in fibre type distribution between RFPs and controls. All RFPs showed fibre atrophy [mean cross-sectional area (CSA) 3300 ± 1100 μm

Original language	English
Pages (from-to)	2074-2081
Journal	Nephrology, Dialysis, Transplantation
Volume	18
DOIs	https://doi.org/10.1093/ndt/gfg325
Publication status	Published - 2003

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title = "Atrophy in non-locomotor muscle in patients with end-stage renal failure",

abstract = "Background. All previous histological studies of skeletal muscles of patients with renal failure have used locomotor muscle biopsies. It is thus unclear to what degree the observed abnormalities are due to the uraemic state and how much is due to disuse. The present study was undertaken to attempt to investigate this question by examining a non-locomotor muscle (rectus abdominis) in patients with end-stage renal failure. Methods. Biopsies from rectus abdominis were obtained from 22 renal failure patients (RFPs) undergoing surgical Tenchkoff catheter implantation for peritoneal dialysis and 20 control subjects undergoing elective abdominal surgery. Histochemical staining of frozen sections and morphometric analysis was used to estimate the proportion of each fibre type, muscle fibre area and capillary density. Myosin heavy chain composition was examined by SDS-PAGE. Results. There were no differences in fibre type distribution between RFPs and controls. All RFPs showed fibre atrophy [mean cross-sectional area (CSA) 3300 ± 1100 μm",

author = "G.K. Sakkas and D. Ball and T.H. Mercer and A.J. Sargeant and K. Tolfrey and P.F. Naish",

year = "2003",

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T1 - Atrophy in non-locomotor muscle in patients with end-stage renal failure

AU - Sakkas, G.K.

AU - Ball, D.

AU - Mercer, T.H.

AU - Sargeant, A.J.

AU - Tolfrey, K.

AU - Naish, P.F.

PY - 2003

Y1 - 2003

N2 - Background. All previous histological studies of skeletal muscles of patients with renal failure have used locomotor muscle biopsies. It is thus unclear to what degree the observed abnormalities are due to the uraemic state and how much is due to disuse. The present study was undertaken to attempt to investigate this question by examining a non-locomotor muscle (rectus abdominis) in patients with end-stage renal failure. Methods. Biopsies from rectus abdominis were obtained from 22 renal failure patients (RFPs) undergoing surgical Tenchkoff catheter implantation for peritoneal dialysis and 20 control subjects undergoing elective abdominal surgery. Histochemical staining of frozen sections and morphometric analysis was used to estimate the proportion of each fibre type, muscle fibre area and capillary density. Myosin heavy chain composition was examined by SDS-PAGE. Results. There were no differences in fibre type distribution between RFPs and controls. All RFPs showed fibre atrophy [mean cross-sectional area (CSA) 3300 ± 1100 μm

AB - Background. All previous histological studies of skeletal muscles of patients with renal failure have used locomotor muscle biopsies. It is thus unclear to what degree the observed abnormalities are due to the uraemic state and how much is due to disuse. The present study was undertaken to attempt to investigate this question by examining a non-locomotor muscle (rectus abdominis) in patients with end-stage renal failure. Methods. Biopsies from rectus abdominis were obtained from 22 renal failure patients (RFPs) undergoing surgical Tenchkoff catheter implantation for peritoneal dialysis and 20 control subjects undergoing elective abdominal surgery. Histochemical staining of frozen sections and morphometric analysis was used to estimate the proportion of each fibre type, muscle fibre area and capillary density. Myosin heavy chain composition was examined by SDS-PAGE. Results. There were no differences in fibre type distribution between RFPs and controls. All RFPs showed fibre atrophy [mean cross-sectional area (CSA) 3300 ± 1100 μm

U2 - 10.1093/ndt/gfg325

DO - 10.1093/ndt/gfg325

M3 - Article

SN - 0931-0509

VL - 18

SP - 2074

EP - 2081

JO - Nephrology, Dialysis, Transplantation

JF - Nephrology, Dialysis, Transplantation

ER -

Atrophy in non-locomotor muscle in patients with end-stage renal failure

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