Automated high-performance liquid chromatographic determination of antipyrine and its main metabolites in plasma, saliva and urine, including 4,4'-dihydroxyantipyrine

M W Teunissen, J E Meerburg-van der Torren, N P Vermeulen, D D Breimer

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

A rapid, selective and sensitive method was developed for the determination of antipyrine and its main metabolites in plasma, saliva and urine by an automated high-performance liquid chromatographic system. Using a MOS-Hypersil reversed-phase column with a phosphate buffer--acetonitrile mobile phase, baseline separation of antipyrine, its metabolites 3-hydroxymethylantipyrine, norantipyrine and 4-hydroxyantipyrine, and the internal standard, phenacetin, was achieved within 6 min. Factors regarding the accuracy and precision of the method and the stability of phase I metabolites during sample preparation are discussed, taking into account certain drawbacks of previously published methods. Based on the same chromatographic system a method was developed for the assay of 4,4'-dihydroxyantipyrine in urine. This compound is an important metabolite of antipyrine in the rat, representing 12.6 +/- 1.8% of the administered dose (n = 18).

Original languageEnglish
Pages (from-to)367-78
Number of pages12
JournalJournal of Chromatography A
Volume278
Issue number2
Publication statusPublished - 9 Dec 1983

Keywords

  • Animals
  • Antipyrine
  • Autoanalysis
  • Chromatography, High Pressure Liquid
  • Humans
  • Hydrolysis
  • Rats
  • Saliva
  • Time Factors
  • Journal Article
  • Research Support, Non-U.S. Gov't

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