Bacterial community dynamics in lait caillé, a traditional product of spontaneous fermentation from Senegal

Anneloes E. Groenenboom, Megan E. Parker, Anne De Vries, Suzette De Groot, Stephanie Zobrist, Kimberly Mansen, Peiman Milani, Remco Kort, Eddy J. Smid, Sijmen E. Schoustra

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Spontaneously fermented food products contain a complex, natural microbial community with potential probiotic activity. The addition of a health-promoting, probiotic bacterium to these products ensures the delivery of that probiotic activity to consumers. Here, we assess the microbial community of a traditional Senegalese milk product produced by spontaneous fermentation, called lait caillé. We produced the lait caillé in a traditional way and added a probiotic starter containing Lactobacillus rhamnosus yoba 2012 to the traditional process. We found various species that are known for their ability to ferment milk, including species from the genera Lactobacillus, Acetobacter, Lactococcus, and Streptococcus. Our results show that the addition of L. rhamnosus to the inoculum, can result in detectable levels of this strain in the final product, ranging between 0.2 and 1 percent of the total bacterial population. Subsequent rounds of fermentation using passive back-slopping without the addition of new L. rhamnosus led to a loss of this strain from the community of fermenting bacteria. Our results suggest that the addition of probiotic strains at every fermentation cycle can enrich the existing complex communities of traditionally fermented lait caillé while traditional bacterial strains remain dominant in the bacterial communities.

Original languageEnglish
Article numbere0215658
JournalPLoS ONE
Volume14
Issue number5
DOIs
Publication statusPublished - 10 May 2019

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Senegal
Probiotics
bacterial communities
Fermentation
probiotics
Lactobacillus rhamnosus
fermentation
microbial communities
Bacteria
Milk
Acetobacter
Lactococcus
health promotion
Starters
fermented foods
bacteria
Streptococcus
Lactobacillus
dairy products
inoculum

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Groenenboom, A. E., Parker, M. E., De Vries, A., De Groot, S., Zobrist, S., Mansen, K., ... Schoustra, S. E. (2019). Bacterial community dynamics in lait caillé, a traditional product of spontaneous fermentation from Senegal. PLoS ONE, 14(5), [e0215658]. https://doi.org/10.1371/journal.pone.0215658
Groenenboom, Anneloes E. ; Parker, Megan E. ; De Vries, Anne ; De Groot, Suzette ; Zobrist, Stephanie ; Mansen, Kimberly ; Milani, Peiman ; Kort, Remco ; Smid, Eddy J. ; Schoustra, Sijmen E. / Bacterial community dynamics in lait caillé, a traditional product of spontaneous fermentation from Senegal. In: PLoS ONE. 2019 ; Vol. 14, No. 5.
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abstract = "Spontaneously fermented food products contain a complex, natural microbial community with potential probiotic activity. The addition of a health-promoting, probiotic bacterium to these products ensures the delivery of that probiotic activity to consumers. Here, we assess the microbial community of a traditional Senegalese milk product produced by spontaneous fermentation, called lait caill{\'e}. We produced the lait caill{\'e} in a traditional way and added a probiotic starter containing Lactobacillus rhamnosus yoba 2012 to the traditional process. We found various species that are known for their ability to ferment milk, including species from the genera Lactobacillus, Acetobacter, Lactococcus, and Streptococcus. Our results show that the addition of L. rhamnosus to the inoculum, can result in detectable levels of this strain in the final product, ranging between 0.2 and 1 percent of the total bacterial population. Subsequent rounds of fermentation using passive back-slopping without the addition of new L. rhamnosus led to a loss of this strain from the community of fermenting bacteria. Our results suggest that the addition of probiotic strains at every fermentation cycle can enrich the existing complex communities of traditionally fermented lait caill{\'e} while traditional bacterial strains remain dominant in the bacterial communities.",
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Groenenboom, AE, Parker, ME, De Vries, A, De Groot, S, Zobrist, S, Mansen, K, Milani, P, Kort, R, Smid, EJ & Schoustra, SE 2019, 'Bacterial community dynamics in lait caillé, a traditional product of spontaneous fermentation from Senegal' PLoS ONE, vol. 14, no. 5, e0215658. https://doi.org/10.1371/journal.pone.0215658

Bacterial community dynamics in lait caillé, a traditional product of spontaneous fermentation from Senegal. / Groenenboom, Anneloes E.; Parker, Megan E.; De Vries, Anne; De Groot, Suzette; Zobrist, Stephanie; Mansen, Kimberly; Milani, Peiman; Kort, Remco; Smid, Eddy J.; Schoustra, Sijmen E.

In: PLoS ONE, Vol. 14, No. 5, e0215658, 10.05.2019.

Research output: Contribution to JournalArticleAcademicpeer-review

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Groenenboom AE, Parker ME, De Vries A, De Groot S, Zobrist S, Mansen K et al. Bacterial community dynamics in lait caillé, a traditional product of spontaneous fermentation from Senegal. PLoS ONE. 2019 May 10;14(5). e0215658. https://doi.org/10.1371/journal.pone.0215658