TY - JOUR
T1 - Barcoding hybrids
T2 - Heterogeneous distribution of Schistosoma haematobium × Schistosoma bovis hybrids across the Senegal River Basin
AU - Boon, Nele A.M.
AU - Van Den Broeck, Frederik
AU - Faye, Djiby
AU - Volckaert, Filip A.M.
AU - Mboup, Souleymane
AU - Polman, Katja
AU - Huyse, Tine
PY - 2018/4/1
Y1 - 2018/4/1
N2 - Hybridization events between Schistosoma species (Digenea, Platyhelminthes) are reported with increasing frequency, largely due to improved access to molecular tools. Nevertheless, little is known about the distribution and frequency of hybrid schistosomes in nature. Screening for hybrids on a large scale is complicated by the need for nuclear and mitochondrial sequence information, precluding a 'simple' barcoding approach. Here we aimed to determine and understand the spatiotemporal distribution of Schistosoma haematobium × Schistosoma bovis hybrids in the Senegal River Basin. From ten villages, distributed over the four main water basins, we genotyped a total of 1236 schistosome larvae collected from human urine samples using a partial mitochondrial cox1 fragment; a subset of 268 parasites was also genotyped using ITS rDNA. Hybrid schistosomes were unevenly distributed, with substantially higher numbers in villages bordering Lac de Guiers than in villages from the Lampsar River and the Middle Valley of the Senegal River. The frequency of hybrids per village was not linked with the prevalence of urinary schistosomiasis in that village. However, we did find a significant positive association between the frequency of hybrids per village and the prevalence of Schistosoma mansoni. We discuss the potential consequences of adopting a barcoding approach when studying hybrids in nature.
AB - Hybridization events between Schistosoma species (Digenea, Platyhelminthes) are reported with increasing frequency, largely due to improved access to molecular tools. Nevertheless, little is known about the distribution and frequency of hybrid schistosomes in nature. Screening for hybrids on a large scale is complicated by the need for nuclear and mitochondrial sequence information, precluding a 'simple' barcoding approach. Here we aimed to determine and understand the spatiotemporal distribution of Schistosoma haematobium × Schistosoma bovis hybrids in the Senegal River Basin. From ten villages, distributed over the four main water basins, we genotyped a total of 1236 schistosome larvae collected from human urine samples using a partial mitochondrial cox1 fragment; a subset of 268 parasites was also genotyped using ITS rDNA. Hybrid schistosomes were unevenly distributed, with substantially higher numbers in villages bordering Lac de Guiers than in villages from the Lampsar River and the Middle Valley of the Senegal River. The frequency of hybrids per village was not linked with the prevalence of urinary schistosomiasis in that village. However, we did find a significant positive association between the frequency of hybrids per village and the prevalence of Schistosoma mansoni. We discuss the potential consequences of adopting a barcoding approach when studying hybrids in nature.
KW - barcoding
KW - hybrids
KW - ITS rDNA
KW - Key wordsHybridization
KW - Schistosoma bovis
KW - Schistosoma haematobium
KW - Senegal
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U2 - 10.1017/S0031182018000525
DO - 10.1017/S0031182018000525
M3 - Article
C2 - 29667570
AN - SCOPUS:85045666970
SN - 0031-1820
VL - 145
SP - 634
EP - 645
JO - Parasitology
JF - Parasitology
IS - 5
ER -