Bending dynamics of fluctuating biopolymers probed by automated high-resolution filament tracking

C. P. Brangwynne, G.H. Koenderink, E. Barry, Z. Dogic, F.C. Mac Kintosh, D. A. Weitz

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Abstract

Microscope images of fluctuating biopolymers contain a wealth of information about their underlying mechanics and dynamics. However, successful extraction of this information requires precise localization of filament position and shape from thousands of noisy images. Here, we present careful measurements of the bending dynamics of filamentous (F-)actin and microtubules at thermal equilibrium with high spatial and temporal resolution using a new, simple but robust, automated image analysis algorithm with subpixel accuracy. We find that slender actin filaments have a persistence length of ∼17 μm, and display a q
Original languageEnglish
Pages (from-to)346-359
Number of pages14
JournalBiophysical Journal
Volume93
Issue number1
DOIs
Publication statusPublished - 1 Jul 2007

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