TY - JOUR
T1 - Binding and potential-triggered release of L-glutamate with molecularly imprinted polypyrrole in neutral pH solutions
AU - Chernov, I.
AU - Greb, H.
AU - Janssen-Bienhold, U.
AU - Parisi, J.
AU - Weiler, R.
AU - von Hauff, E.L.
PY - 2014
Y1 - 2014
N2 - Molecularly imprinted polymers (MIPs) are interesting for potential-regulated trafficking of molecules. In this study, we investigate the binding properties of l-glutamate (Glu) in molecularly imprinted polypyrrole (MIPPy) in neutral pH solutions. We prepared MIPPy by electrochemically depositing Glu-doped polypyrrole and subsequently overoxidizing the polymer. By means of immunofluorescence microscopy analysis of MIPPy using specific anti-Glu antibodies, we demonstrate binding of Glu in MIPPy. Visual imaging of the samples allows us to easily discern between selective and non-selective interactions. Significant fluorescence is observed from MIPPy incubated in solutions containing Glu, while control MIPPy samples imprinted with Cl
AB - Molecularly imprinted polymers (MIPs) are interesting for potential-regulated trafficking of molecules. In this study, we investigate the binding properties of l-glutamate (Glu) in molecularly imprinted polypyrrole (MIPPy) in neutral pH solutions. We prepared MIPPy by electrochemically depositing Glu-doped polypyrrole and subsequently overoxidizing the polymer. By means of immunofluorescence microscopy analysis of MIPPy using specific anti-Glu antibodies, we demonstrate binding of Glu in MIPPy. Visual imaging of the samples allows us to easily discern between selective and non-selective interactions. Significant fluorescence is observed from MIPPy incubated in solutions containing Glu, while control MIPPy samples imprinted with Cl
UR - https://www.scopus.com/pages/publications/84905585975
UR - https://www.scopus.com/inward/citedby.url?scp=84905585975&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2014.06.030
DO - 10.1016/j.snb.2014.06.030
M3 - Article
SN - 0925-4005
VL - 203
SP - 327
EP - 332
JO - Sensors and Actuators. B, Chemical
JF - Sensors and Actuators. B, Chemical
ER -
