Bioactivation of N-substituted N'-(4-imidazole-ethyl)thioureas by human FMO1 and FMO3

R.C.A. Onderwater, A.E. Rettie, J.N.M. Commandeur, N.P.E. Vermeulen

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Enzyme kinetic parameters of the bioactivation of thiourea-containing compounds by human flavin-containing monooxygenase enzymes (FMOs) FMO1 and FMO3 were investigated. A microtitre-based adaptation of methodology described for the thiourea-dependent oxidation of thiocholine was used to determine the turnover of thiourea-containing compounds by human FMO1 and FMO3. The results show that major differences in enzyme kinetic parameters for N-substituted N′-(4-imidazole-ethyl)thiourea exist between human FMO3 and human FMO1. Whereas K
Original languageEnglish
Pages (from-to)645-57
JournalXenobiotica
Volume36
Issue number7
DOIs
Publication statusPublished - 2006

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