Bioactivation of N-substituted N'-(4-imidazole-ethyl)thioureas by human FMO1 and FMO3

R.C.A. Onderwater; A.E. Rettie; J.N.M. Commandeur; N.P.E. Vermeulen

doi:10.1080/00498250500354329

Bioactivation of N-substituted N'-(4-imidazole-ethyl)thioureas by human FMO1 and FMO3

R.C.A. Onderwater, A.E. Rettie, J.N.M. Commandeur, N.P.E. Vermeulen

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Enzyme kinetic parameters of the bioactivation of thiourea-containing compounds by human flavin-containing monooxygenase enzymes (FMOs) FMO1 and FMO3 were investigated. A microtitre-based adaptation of methodology described for the thiourea-dependent oxidation of thiocholine was used to determine the turnover of thiourea-containing compounds by human FMO1 and FMO3. The results show that major differences in enzyme kinetic parameters for N-substituted N′-(4-imidazole-ethyl)thiourea exist between human FMO3 and human FMO1. Whereas K

Original language	English
Pages (from-to)	645-57
Journal	Xenobiotica
Volume	36
Issue number	7
DOIs	https://doi.org/10.1080/00498250500354329
Publication status	Published - 2006

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title = "Bioactivation of N-substituted N'-(4-imidazole-ethyl)thioureas by human FMO1 and FMO3",

abstract = "Enzyme kinetic parameters of the bioactivation of thiourea-containing compounds by human flavin-containing monooxygenase enzymes (FMOs) FMO1 and FMO3 were investigated. A microtitre-based adaptation of methodology described for the thiourea-dependent oxidation of thiocholine was used to determine the turnover of thiourea-containing compounds by human FMO1 and FMO3. The results show that major differences in enzyme kinetic parameters for N-substituted N′-(4-imidazole-ethyl)thiourea exist between human FMO3 and human FMO1. Whereas K",

author = "R.C.A. Onderwater and A.E. Rettie and J.N.M. Commandeur and N.P.E. Vermeulen",

year = "2006",

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T1 - Bioactivation of N-substituted N'-(4-imidazole-ethyl)thioureas by human FMO1 and FMO3

AU - Onderwater, R.C.A.

AU - Rettie, A.E.

AU - Commandeur, J.N.M.

AU - Vermeulen, N.P.E.

PY - 2006

Y1 - 2006

N2 - Enzyme kinetic parameters of the bioactivation of thiourea-containing compounds by human flavin-containing monooxygenase enzymes (FMOs) FMO1 and FMO3 were investigated. A microtitre-based adaptation of methodology described for the thiourea-dependent oxidation of thiocholine was used to determine the turnover of thiourea-containing compounds by human FMO1 and FMO3. The results show that major differences in enzyme kinetic parameters for N-substituted N′-(4-imidazole-ethyl)thiourea exist between human FMO3 and human FMO1. Whereas K

AB - Enzyme kinetic parameters of the bioactivation of thiourea-containing compounds by human flavin-containing monooxygenase enzymes (FMOs) FMO1 and FMO3 were investigated. A microtitre-based adaptation of methodology described for the thiourea-dependent oxidation of thiocholine was used to determine the turnover of thiourea-containing compounds by human FMO1 and FMO3. The results show that major differences in enzyme kinetic parameters for N-substituted N′-(4-imidazole-ethyl)thiourea exist between human FMO3 and human FMO1. Whereas K

U2 - 10.1080/00498250500354329

DO - 10.1080/00498250500354329

M3 - Article

VL - 36

SP - 645

EP - 657

JO - Xenobiotica

JF - Xenobiotica

SN - 0049-8254

IS - 7

ER -

Bioactivation of N-substituted N'-(4-imidazole-ethyl)thioureas by human FMO1 and FMO3

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