TY - JOUR
T1 - Biogenesis of inner membrane proteins in Escherichia coli
AU - Luirink, S.
AU - von Heijne, G.
AU - Houben, E.N.G.
AU - de Gier, J.-W.
PY - 2005
Y1 - 2005
N2 - Gram-negative bacteria such as Escherichia coli are surrounded by two membranes, the inner membrane and the outer membrane. The biogenesis of most inner membrane proteins (IMPs), typical α-helical proteins, appears to follow a partly conserved cotranslational pathway. Targeting involves a relatively simple signal recognition particle (SRP) and SRP-receptor. Insertion of most IMPs into the membrane occurs via the Sec-translocon, which is also used for the vectorial transport of secretory proteins. Similar to eukaryotic systems, little is known about the later stages of biogenesis of IMPs, the folding and assembly in the lipid bilayer. Recently, YidC has been identified as a factor that assists in the integration, folding, and assembly of IMPs both in association with the Sec-translocon and separately. This review deals mainly with recent structural and biochemical data from various experimental systems that offer new insight into the different stages of biogenesis of E. coli IMPs. Copyright © 2005 by Annual Reviews. All rights reserved.
AB - Gram-negative bacteria such as Escherichia coli are surrounded by two membranes, the inner membrane and the outer membrane. The biogenesis of most inner membrane proteins (IMPs), typical α-helical proteins, appears to follow a partly conserved cotranslational pathway. Targeting involves a relatively simple signal recognition particle (SRP) and SRP-receptor. Insertion of most IMPs into the membrane occurs via the Sec-translocon, which is also used for the vectorial transport of secretory proteins. Similar to eukaryotic systems, little is known about the later stages of biogenesis of IMPs, the folding and assembly in the lipid bilayer. Recently, YidC has been identified as a factor that assists in the integration, folding, and assembly of IMPs both in association with the Sec-translocon and separately. This review deals mainly with recent structural and biochemical data from various experimental systems that offer new insight into the different stages of biogenesis of E. coli IMPs. Copyright © 2005 by Annual Reviews. All rights reserved.
U2 - 10.1146/annurev.micro.59.030804.121246
DO - 10.1146/annurev.micro.59.030804.121246
M3 - Article
VL - 59
SP - 329
EP - 355
JO - Annual Review of Microbiology
JF - Annual Review of Microbiology
SN - 0066-4227
ER -