Biogenesis of inner membrane proteins in Escherichia coli

S. Luirink, G. von Heijne, E.N.G. Houben, J.-W. de Gier

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Gram-negative bacteria such as Escherichia coli are surrounded by two membranes, the inner membrane and the outer membrane. The biogenesis of most inner membrane proteins (IMPs), typical α-helical proteins, appears to follow a partly conserved cotranslational pathway. Targeting involves a relatively simple signal recognition particle (SRP) and SRP-receptor. Insertion of most IMPs into the membrane occurs via the Sec-translocon, which is also used for the vectorial transport of secretory proteins. Similar to eukaryotic systems, little is known about the later stages of biogenesis of IMPs, the folding and assembly in the lipid bilayer. Recently, YidC has been identified as a factor that assists in the integration, folding, and assembly of IMPs both in association with the Sec-translocon and separately. This review deals mainly with recent structural and biochemical data from various experimental systems that offer new insight into the different stages of biogenesis of E. coli IMPs. Copyright © 2005 by Annual Reviews. All rights reserved.
Original languageEnglish
Pages (from-to)329-355
Number of pages17
JournalAnnual Review of Microbiology
Volume59
DOIs
Publication statusPublished - 2005

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Membrane Proteins
Escherichia coli
Membranes
Signal Recognition Particle
Protein Folding
Lipid Bilayers
Gram-Negative Bacteria
Carrier Proteins
Proteins

Cite this

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title = "Biogenesis of inner membrane proteins in Escherichia coli",
abstract = "Gram-negative bacteria such as Escherichia coli are surrounded by two membranes, the inner membrane and the outer membrane. The biogenesis of most inner membrane proteins (IMPs), typical α-helical proteins, appears to follow a partly conserved cotranslational pathway. Targeting involves a relatively simple signal recognition particle (SRP) and SRP-receptor. Insertion of most IMPs into the membrane occurs via the Sec-translocon, which is also used for the vectorial transport of secretory proteins. Similar to eukaryotic systems, little is known about the later stages of biogenesis of IMPs, the folding and assembly in the lipid bilayer. Recently, YidC has been identified as a factor that assists in the integration, folding, and assembly of IMPs both in association with the Sec-translocon and separately. This review deals mainly with recent structural and biochemical data from various experimental systems that offer new insight into the different stages of biogenesis of E. coli IMPs. Copyright {\circledC} 2005 by Annual Reviews. All rights reserved.",
author = "S. Luirink and {von Heijne}, G. and E.N.G. Houben and {de Gier}, J.-W.",
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Biogenesis of inner membrane proteins in Escherichia coli. / Luirink, S.; von Heijne, G.; Houben, E.N.G.; de Gier, J.-W.

In: Annual Review of Microbiology, Vol. 59, 2005, p. 329-355.

Research output: Contribution to JournalArticleAcademicpeer-review

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AU - Luirink, S.

AU - von Heijne, G.

AU - Houben, E.N.G.

AU - de Gier, J.-W.

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AB - Gram-negative bacteria such as Escherichia coli are surrounded by two membranes, the inner membrane and the outer membrane. The biogenesis of most inner membrane proteins (IMPs), typical α-helical proteins, appears to follow a partly conserved cotranslational pathway. Targeting involves a relatively simple signal recognition particle (SRP) and SRP-receptor. Insertion of most IMPs into the membrane occurs via the Sec-translocon, which is also used for the vectorial transport of secretory proteins. Similar to eukaryotic systems, little is known about the later stages of biogenesis of IMPs, the folding and assembly in the lipid bilayer. Recently, YidC has been identified as a factor that assists in the integration, folding, and assembly of IMPs both in association with the Sec-translocon and separately. This review deals mainly with recent structural and biochemical data from various experimental systems that offer new insight into the different stages of biogenesis of E. coli IMPs. Copyright © 2005 by Annual Reviews. All rights reserved.

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