Biosensor immunoassays for the detection of bisphenol A

G.R. Marchesini, E.P. meulenberg, W. Haasnoot, H. Irth

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Bisphenol A (BPA) is a xenoestrogen found in the environment, in consequence, for the biosensor detection of BPA we raised antibodies (polyclonal (PAbs) and monoclonal (MAbs)) against a structural analogue of BPA, 4,4 bis-(4-hydroxyphenyl) valeric acid (BVA). The kinetics of the MAb-BPA interaction were evaluated and the MAb providing the highest affinity was directly immobilized onto the sensor chip surface to evaluate a direct assay. Afterwards, the performance of the MAbs and the PAbs was compared in an inhibition assay using a BVA-coated chip. The highest sensitivity (limit of detection (LOD) of 0.4 μg L
Original languageEnglish
Pages (from-to)37-45
JournalAnalytica Chimica Acta
Volume528
Issue number1
DOIs
Publication statusPublished - 2005

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