Blood-borne circadian signal stimulates daily oscillations in actin dynamics and SRF activity

Alan Gerber, Cyril Esnault, Gregory Aubert, Richard Treisman, François Pralong, Ueli Schibler

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

In peripheral tissues circadian gene expression can be driven either by local oscillators or by cyclic systemic cues controlled by the master clock in the brain's suprachiasmatic nucleus. In the latter case, systemic signals can activate immediate early transcription factors (IETFs) and thereby control rhythmic transcription. In order to identify IETFs induced by diurnal blood-borne signals, we developed an unbiased experimental strategy, dubbed Synthetic TAndem Repeat PROMoter (STAR-PROM) screening. This technique relies on the observation that most transcription factor binding sites exist at a relatively high frequency in random DNA sequences. Using STAR-PROM we identified serum response factor (SRF) as an IETF responding to oscillating signaling proteins present in human and rodent sera. Our data suggest that in mouse liver SRF is regulated via dramatic diurnal changes of actin dynamics, leading to the rhythmic translocation of the SRF coactivator Myocardin-related transcription factor-B (MRTF-B) into the nucleus.

Original languageEnglish
Pages (from-to)492-503
Number of pages12
JournalCell
Volume152
Issue number3
DOIs
Publication statusPublished - 31 Jan 2013

Fingerprint

Serum Response Factor
Actins
Blood
Transcription Factors
Tandem Repeat Sequences
Suprachiasmatic Nucleus
DNA sequences
Transcription
Gene expression
Liver
Cues
Clocks
Rodentia
Brain
Screening
Binding Sites
Tissue
Gene Expression
Serum
Proteins

Cite this

Gerber, Alan ; Esnault, Cyril ; Aubert, Gregory ; Treisman, Richard ; Pralong, François ; Schibler, Ueli. / Blood-borne circadian signal stimulates daily oscillations in actin dynamics and SRF activity. In: Cell. 2013 ; Vol. 152, No. 3. pp. 492-503.
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Gerber, A, Esnault, C, Aubert, G, Treisman, R, Pralong, F & Schibler, U 2013, 'Blood-borne circadian signal stimulates daily oscillations in actin dynamics and SRF activity' Cell, vol. 152, no. 3, pp. 492-503. https://doi.org/10.1016/j.cell.2012.12.027

Blood-borne circadian signal stimulates daily oscillations in actin dynamics and SRF activity. / Gerber, Alan; Esnault, Cyril; Aubert, Gregory; Treisman, Richard; Pralong, François; Schibler, Ueli.

In: Cell, Vol. 152, No. 3, 31.01.2013, p. 492-503.

Research output: Contribution to JournalArticleAcademicpeer-review

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