BMP-2 incorporated into a biomimetic coating on 3D-printed titanium scaffold promotes mandibular bicortical bone formation in a beagle dog model

Yifei Gu, Yiwen Liu, Reinhilde Jacobs, Lingfei Wei*, Yi Sun, Lei Tian, Yuelian Liu, Constantinus Politis

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Biocompatibility and osteoconductivity of porous scaffolds can be improved through coating technique by creating micro- and nanostructures on the surfaces. In this study, a biomimetic approach was used to construct a bone morphogenetic protein-2 (BMP-2) integrated calcium phosphate (CaP) coating on the surface of porous titanium scaffolds. In vitro characterizations showed that the scaffolds had mechanical properties comparable to those of the human cortical bone. The biomimetic coating has a nano-scaled surface structure, which releases BMP-2 in a stable and effective manner. Bicortical bone defects were prepared in the bilateral mandibles of beagle dogs, and the coated and uncoated scaffolds were fixed in the bone defects for 8 weeks. Based on micro-CT and histological analysis results, the biomimetic coating significantly promoted bone formation in the titanium scaffold. The coated scaffold group had significantly higher bone volume fraction, bone area fraction, and bone-to-implant contact results than the uncoated scaffold group. Besides, hematological indexes and histopathology results of the visceral organs confirmed the scaffold and coating's biocompatibility. Accordingly, the current study demonstrates that BMP-2 integrated biomimetic CaP coating can significantly promote bone formation in porous titanium scaffolds and benefit the healing process of the bicortical mandibular defect in beagle dogs.

Original languageEnglish
Article number111849
Pages (from-to)1-10
Number of pages10
JournalMaterials and Design
Volume228
Early online date21 Mar 2023
DOIs
Publication statusPublished - Apr 2023

Bibliographical note

Funding Information:
This study was supported by the Xi’an Science and Technology Plan (No.22YXYJ0156), Natural Science Foundation of Shandong Province (No. ZR2020QH162 ), and National Natural Science Foundation of China (No. 82100965 ).

Funding Information:
This study was supported by the Xi'an Science and Technology Plan (No.22YXYJ0156), Natural Science Foundation of Shandong Province (No. ZR2020QH162), and National Natural Science Foundation of China (No.82100965).

Publisher Copyright:
© 2023 The Authors

Funding

This study was supported by the Xi’an Science and Technology Plan (No.22YXYJ0156), Natural Science Foundation of Shandong Province (No. ZR2020QH162 ), and National Natural Science Foundation of China (No. 82100965 ). This study was supported by the Xi'an Science and Technology Plan (No.22YXYJ0156), Natural Science Foundation of Shandong Province (No. ZR2020QH162), and National Natural Science Foundation of China (No.82100965).

FundersFunder number
Xi'an Science and Technology Plan
Xi’an Science and Technology Plan22YXYJ0156
National Natural Science Foundation of China82100965
National Natural Science Foundation of China
Natural Science Foundation of Shandong ProvinceZR2020QH162
Natural Science Foundation of Shandong Province

    Keywords

    • 3D-printing
    • Biomimetics
    • Bone morphogenetic protein 2
    • Calcium phosphate
    • Titanium alloy

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