Abstract
Biocompatibility and osteoconductivity of porous scaffolds can be improved through coating technique by creating micro- and nanostructures on the surfaces. In this study, a biomimetic approach was used to construct a bone morphogenetic protein-2 (BMP-2) integrated calcium phosphate (CaP) coating on the surface of porous titanium scaffolds. In vitro characterizations showed that the scaffolds had mechanical properties comparable to those of the human cortical bone. The biomimetic coating has a nano-scaled surface structure, which releases BMP-2 in a stable and effective manner. Bicortical bone defects were prepared in the bilateral mandibles of beagle dogs, and the coated and uncoated scaffolds were fixed in the bone defects for 8 weeks. Based on micro-CT and histological analysis results, the biomimetic coating significantly promoted bone formation in the titanium scaffold. The coated scaffold group had significantly higher bone volume fraction, bone area fraction, and bone-to-implant contact results than the uncoated scaffold group. Besides, hematological indexes and histopathology results of the visceral organs confirmed the scaffold and coating's biocompatibility. Accordingly, the current study demonstrates that BMP-2 integrated biomimetic CaP coating can significantly promote bone formation in porous titanium scaffolds and benefit the healing process of the bicortical mandibular defect in beagle dogs.
Original language | English |
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Article number | 111849 |
Pages (from-to) | 1-10 |
Number of pages | 10 |
Journal | Materials and Design |
Volume | 228 |
Early online date | 21 Mar 2023 |
DOIs | |
Publication status | Published - Apr 2023 |
Bibliographical note
Funding Information:This study was supported by the Xi’an Science and Technology Plan (No.22YXYJ0156), Natural Science Foundation of Shandong Province (No. ZR2020QH162 ), and National Natural Science Foundation of China (No. 82100965 ).
Funding Information:
This study was supported by the Xi'an Science and Technology Plan (No.22YXYJ0156), Natural Science Foundation of Shandong Province (No. ZR2020QH162), and National Natural Science Foundation of China (No.82100965).
Publisher Copyright:
© 2023 The Authors
Funding
This study was supported by the Xi’an Science and Technology Plan (No.22YXYJ0156), Natural Science Foundation of Shandong Province (No. ZR2020QH162 ), and National Natural Science Foundation of China (No. 82100965 ). This study was supported by the Xi'an Science and Technology Plan (No.22YXYJ0156), Natural Science Foundation of Shandong Province (No. ZR2020QH162), and National Natural Science Foundation of China (No.82100965).
Funders | Funder number |
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Xi'an Science and Technology Plan | |
Xi’an Science and Technology Plan | 22YXYJ0156 |
National Natural Science Foundation of China | 82100965 |
National Natural Science Foundation of China | |
Natural Science Foundation of Shandong Province | ZR2020QH162 |
Natural Science Foundation of Shandong Province |
Keywords
- 3D-printing
- Biomimetics
- Bone morphogenetic protein 2
- Calcium phosphate
- Titanium alloy