BMP2-coprecipitated calcium phosphate granules enhance osteoinductivity of deproteinized bovine bone, and bone formation during critical-sized bone defect healing

T. Liu, Y. Zheng, G. Wu, D. Wismeijer, J.L. Pathak, Y. Liu

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Most materials used clinically for filling critical-sized bone defects (CSBD), such as deproteinized bovine bone (DBB), lack osteoinductivity so that their therapeutic effects are far from satisfactory. The effect of bone morphogenic protein 2 (BMP2)-coprecipitated biomimetic calcium phosphate granules (BMP2-cop.BioCaP) on osteoinduction of DBB graft(s) during CSBD healing is still unknown. We investigated whether BMP2-cop.BioCaP affects the osteoinductivity of DBB, bone formation, and foreign body reaction during CSBD healing. DBB + BMP2-cop.BioCaP, DBB, DBB + BMP2, DBB + BioCaP, and autologous bone grafts were implanted in the CSBD of sheep. Bone formation, DBB/BioCaP degradability, foreign body reaction, and osteoinductivity of DBB were analyzed histologically and histomorphometrically at week 4 and 8. Combination of BMP2-cop.BioCaP and DBB healed CSBD as effectively as autologous bone grafts. About 95% of the BMP2-cop.BioCaP had been degraded and replaced by new bone at week 8 in the DBB + BMP2-cop.BioCaP-group. Foreign body reaction was reduced in the DBB + BMP2-cop.BioCaP-group compared to the other groups. The independent use of the BMP2-cop.BioCaP did not achieve a satisfactory bone repair. In conclusion, the BMP2-cop.BioCaP showed good degradability and biocompatibility, and enhanced osteoinductivity of DBB during CSBD healing in sheep, suggesting BMP2-cop.BioCaP as a potential osteoinducer to enhance the therapeutic effects of the graft materials in clinic.

Original languageEnglish
Article number41800
Number of pages12
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 31 Jan 2017

Bibliographical note

With supplementary dataset

Funding

The authors would like to thank Prof. Matthias Brueik for proof reading the manuscript. Dr. Y. Liu acknowledges financial support from the Osteology grant (2008-015) and KNAW grant (11CDP011). Dr. T. Liu acknowledges financial support rom Zhejiang Province TCM Science and Technology Plan (Grant No. 2014ZA064), Zhejiang Provincial Natural Science Foundation of China (Grant No. LQ15H140001), and National Natural Science Foundation of China (Grant No. 81500891).

FundersFunder number
Zhejiang Province TCM Science and Technology Plan2014ZA064
Zhejiang Provincial Natural Science Foundation of ChinaLQ15H140001
Koninklijke Nederlandse Akademie van Wetenschappen11CDP011
National Natural Science Foundation of China81500891

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