Bovine lactoferrin and lactoferrin peptides affect endometrial and cervical cancer cell lines

Diana A. Ramírez-Sánchez; Izamar G. Arredondo-Beltrán; Adrián Canizalez-Roman; Héctor Flores-Villaseñor; Kamran Nazmi; Jan G.M. Bolscher; Nidia León-Sicairos

doi:10.1139/bcb-2020-0074

Bovine lactoferrin and lactoferrin peptides affect endometrial and cervical cancer cell lines

Diana A. Ramírez-Sánchez, Izamar G. Arredondo-Beltrán, Adrián Canizalez-Roman, Héctor Flores-Villaseñor, Kamran Nazmi, Jan G.M. Bolscher, Nidia León-Sicairos^*

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Cervical, uterine, and ovarian cancers are the most common malignancies of the female genital tract worldwide. Despite advances in prevention, early diagnosis, effective screening, and treatment programs, mortality remains high. Consequently, it is important to search for new treatments. The activity of bovine lactoferrin (bLF) and LF peptides against several types of cancer has been studied; however, only a few studies report the effect of bLF and LF peptides against cervical and endometrial cancers. In this study, we explored the effect of bLF as well as LF chimera and its constituent peptides LFcin17-30 and LFampin265-284 on the viability of cervical (HeLa, SiHa) and endometrial (KLE, HEC-1A) cancer cell lines. Cell proliferation was quantified with an MTT assay, cell morphological changes and damage were determined by Giemsa R 488 Annexin V and propidium iodide staining. Additionally, the effect of combinations of bLF and LF peptides with cisplatin was assessed. bLF and LF peptides inhibited the proliferation of uterine cancer cells and caused cellular morphological changes and damage to cell monolayers. bLF induced apoptosis, LFcin17-30 and LFampin265-284 induced apoptosis and necrosis, and LF chimera induced necrosis. Additionally, bLF and LF chimera showed an additive interaction with cisplatin against uterine cancer cells.

Original language	English
Pages (from-to)	149-158
Number of pages	10
Journal	Biochemistry and Cell Biology
Volume	99
Issue number	1
Early online date	12 Dec 2020
DOIs	https://doi.org/10.1139/bcb-2020-0074
Publication status	Published - Feb 2021

Bibliographical note

Funding Information:
This work was supported by grants from CONACYT (CB-2014-236546) and PROFAPI-UAS (2014/105, 2015/141). We thank Erika Acosta Smith and Katherine Herrera-Bernal for their efficient technical support. The authors have no conflicts of interest to declare.

Publisher Copyright:
© The author(s) or their institution(s).

Funding

This work was supported by grants from CONACYT (CB-2014-236546) and PROFAPI-UAS (2014/105, 2015/141). We thank Erika Acosta Smith and Katherine Herrera-Bernal for their efficient technical support. The authors have no conflicts of interest to declare.

Keywords

Apoptosis
Cancer
Lactoferrin
Lactoferrin peptides

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1139/bcb-2020-0074

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title = "Bovine lactoferrin and lactoferrin peptides affect endometrial and cervical cancer cell lines",

abstract = "Cervical, uterine, and ovarian cancers are the most common malignancies of the female genital tract worldwide. Despite advances in prevention, early diagnosis, effective screening, and treatment programs, mortality remains high. Consequently, it is important to search for new treatments. The activity of bovine lactoferrin (bLF) and LF peptides against several types of cancer has been studied; however, only a few studies report the effect of bLF and LF peptides against cervical and endometrial cancers. In this study, we explored the effect of bLF as well as LF chimera and its constituent peptides LFcin17-30 and LFampin265-284 on the viability of cervical (HeLa, SiHa) and endometrial (KLE, HEC-1A) cancer cell lines. Cell proliferation was quantified with an MTT assay, cell morphological changes and damage were determined by Giemsa R 488 Annexin V and propidium iodide staining. Additionally, the effect of combinations of bLF and LF peptides with cisplatin was assessed. bLF and LF peptides inhibited the proliferation of uterine cancer cells and caused cellular morphological changes and damage to cell monolayers. bLF induced apoptosis, LFcin17-30 and LFampin265-284 induced apoptosis and necrosis, and LF chimera induced necrosis. Additionally, bLF and LF chimera showed an additive interaction with cisplatin against uterine cancer cells.",

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note = "Funding Information: This work was supported by grants from CONACYT (CB-2014-236546) and PROFAPI-UAS (2014/105, 2015/141). We thank Erika Acosta Smith and Katherine Herrera-Bernal for their efficient technical support. The authors have no conflicts of interest to declare. Publisher Copyright: {\textcopyright} The author(s) or their institution(s).",

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doi = "10.1139/bcb-2020-0074",

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AU - Ramírez-Sánchez, Diana A.

AU - Arredondo-Beltrán, Izamar G.

AU - Canizalez-Roman, Adrián

AU - Flores-Villaseñor, Héctor

AU - Nazmi, Kamran

AU - Bolscher, Jan G.M.

AU - León-Sicairos, Nidia

N1 - Funding Information: This work was supported by grants from CONACYT (CB-2014-236546) and PROFAPI-UAS (2014/105, 2015/141). We thank Erika Acosta Smith and Katherine Herrera-Bernal for their efficient technical support. The authors have no conflicts of interest to declare. Publisher Copyright: © The author(s) or their institution(s).

PY - 2021/2

Y1 - 2021/2

N2 - Cervical, uterine, and ovarian cancers are the most common malignancies of the female genital tract worldwide. Despite advances in prevention, early diagnosis, effective screening, and treatment programs, mortality remains high. Consequently, it is important to search for new treatments. The activity of bovine lactoferrin (bLF) and LF peptides against several types of cancer has been studied; however, only a few studies report the effect of bLF and LF peptides against cervical and endometrial cancers. In this study, we explored the effect of bLF as well as LF chimera and its constituent peptides LFcin17-30 and LFampin265-284 on the viability of cervical (HeLa, SiHa) and endometrial (KLE, HEC-1A) cancer cell lines. Cell proliferation was quantified with an MTT assay, cell morphological changes and damage were determined by Giemsa R 488 Annexin V and propidium iodide staining. Additionally, the effect of combinations of bLF and LF peptides with cisplatin was assessed. bLF and LF peptides inhibited the proliferation of uterine cancer cells and caused cellular morphological changes and damage to cell monolayers. bLF induced apoptosis, LFcin17-30 and LFampin265-284 induced apoptosis and necrosis, and LF chimera induced necrosis. Additionally, bLF and LF chimera showed an additive interaction with cisplatin against uterine cancer cells.

AB - Cervical, uterine, and ovarian cancers are the most common malignancies of the female genital tract worldwide. Despite advances in prevention, early diagnosis, effective screening, and treatment programs, mortality remains high. Consequently, it is important to search for new treatments. The activity of bovine lactoferrin (bLF) and LF peptides against several types of cancer has been studied; however, only a few studies report the effect of bLF and LF peptides against cervical and endometrial cancers. In this study, we explored the effect of bLF as well as LF chimera and its constituent peptides LFcin17-30 and LFampin265-284 on the viability of cervical (HeLa, SiHa) and endometrial (KLE, HEC-1A) cancer cell lines. Cell proliferation was quantified with an MTT assay, cell morphological changes and damage were determined by Giemsa R 488 Annexin V and propidium iodide staining. Additionally, the effect of combinations of bLF and LF peptides with cisplatin was assessed. bLF and LF peptides inhibited the proliferation of uterine cancer cells and caused cellular morphological changes and damage to cell monolayers. bLF induced apoptosis, LFcin17-30 and LFampin265-284 induced apoptosis and necrosis, and LF chimera induced necrosis. Additionally, bLF and LF chimera showed an additive interaction with cisplatin against uterine cancer cells.

KW - Apoptosis

KW - Cancer

KW - Lactoferrin

KW - Lactoferrin peptides

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SN - 0829-8211

VL - 99

SP - 149

EP - 158

JO - Biochemistry and Cell Biology

JF - Biochemistry and Cell Biology

IS - 1

ER -

Bovine lactoferrin and lactoferrin peptides affect endometrial and cervical cancer cell lines

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

Persistent URL (handle)

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