Bringing third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tissue

Laura M.G. Van Huizen, Daniëlle Seinstra, Chris Dickhoff, Teodora Radonic, Idris Bahce, Frank Van Mourik, Jouke T. Annema, Johannes M.A. Daniels, Wim Jan P. Van Boven, Marie Louise Groot

Research output: Chapter in Book / Report / Conference proceedingConference contributionAcademicpeer-review

Abstract

Third and second harmonic generation (THG/SHG) microscopy is a novel imaging technique that has been suggested as a promising clinical tool, mainly for cancer diagnosis. Here, we test for the first time a compact, transportable ex-vivo THG/SHG microscope (Tritos Diagnostics B.V.) for clinical use. In lung surgery, immediate feedback on the nature of the excised tissue is important. We will present the first results of an ongoing study in which we image both lung tumor tissue immediately after excision and frozen section biopsies, and compare the images to the golden standard histology. Prior to this study, we performed a set of experiments collecting 3D images of mouse lung tissue to show that the combination of THG and SHG microscopy reveals key lung morphology components.

Original languageEnglish
Title of host publicationMedical Laser Applications and Laser-Tissue Interactions IX
EditorsLothar D. Lilge, Carsten M. Philipp
PublisherSPIE
ISBN (Electronic)9781510628519
DOIs
Publication statusPublished - 22 Jul 2019
EventMedical Laser Applications and Laser-Tissue Interactions IX 2019 - Munich, Germany
Duration: 23 Jun 201924 Jun 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11079
ISSN (Print)1605-7422

Conference

ConferenceMedical Laser Applications and Laser-Tissue Interactions IX 2019
CountryGermany
CityMunich
Period23/06/1924/06/19

Fingerprint

Harmonic generation
lungs
Microscopy
harmonic generations
Microscopic examination
Tissue
microscopy
lung morphology
Lung
Histology
histology
Biopsy
surgery
imaging techniques
Surgery
mice
Tumors
Frozen Sections
Microscopes
tumors

Keywords

  • Clinic
  • Lung tissue
  • Second harmonic generation microscopy
  • Third harmonic generation microscopy
  • Validation

Cite this

Van Huizen, L. M. G., Seinstra, D., Dickhoff, C., Radonic, T., Bahce, I., Van Mourik, F., ... Groot, M. L. (2019). Bringing third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tissue. In L. D. Lilge, & C. M. Philipp (Eds.), Medical Laser Applications and Laser-Tissue Interactions IX [110790J] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11079). SPIE. https://doi.org/10.1117/12.2527899
Van Huizen, Laura M.G. ; Seinstra, Daniëlle ; Dickhoff, Chris ; Radonic, Teodora ; Bahce, Idris ; Van Mourik, Frank ; Annema, Jouke T. ; Daniels, Johannes M.A. ; Van Boven, Wim Jan P. ; Groot, Marie Louise. / Bringing third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tissue. Medical Laser Applications and Laser-Tissue Interactions IX. editor / Lothar D. Lilge ; Carsten M. Philipp. SPIE, 2019. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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Van Huizen, LMG, Seinstra, D, Dickhoff, C, Radonic, T, Bahce, I, Van Mourik, F, Annema, JT, Daniels, JMA, Van Boven, WJP & Groot, ML 2019, Bringing third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tissue. in LD Lilge & CM Philipp (eds), Medical Laser Applications and Laser-Tissue Interactions IX., 110790J, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11079, SPIE, Medical Laser Applications and Laser-Tissue Interactions IX 2019, Munich, Germany, 23/06/19. https://doi.org/10.1117/12.2527899

Bringing third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tissue. / Van Huizen, Laura M.G.; Seinstra, Daniëlle; Dickhoff, Chris; Radonic, Teodora; Bahce, Idris; Van Mourik, Frank; Annema, Jouke T.; Daniels, Johannes M.A.; Van Boven, Wim Jan P.; Groot, Marie Louise.

Medical Laser Applications and Laser-Tissue Interactions IX. ed. / Lothar D. Lilge; Carsten M. Philipp. SPIE, 2019. 110790J (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11079).

Research output: Chapter in Book / Report / Conference proceedingConference contributionAcademicpeer-review

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Van Huizen LMG, Seinstra D, Dickhoff C, Radonic T, Bahce I, Van Mourik F et al. Bringing third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tissue. In Lilge LD, Philipp CM, editors, Medical Laser Applications and Laser-Tissue Interactions IX. SPIE. 2019. 110790J. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2527899