Cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo 3 solubilized in dodecyl-maltoside

Amer H. Asseri; Albert Godoy-Hernandez; Hojjat Ghasemi Goojani; Holger Lill; Junshi Sakamoto; Duncan G.G. McMillan; Dirk Bald

doi:10.1038/s41598-021-87354-0

Cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo ₃ solubilized in dodecyl-maltoside

Amer H. Asseri, Albert Godoy-Hernandez, Hojjat Ghasemi Goojani, Holger Lill, Junshi Sakamoto, Duncan G.G. McMillan, Dirk Bald^*

^*Corresponding author for this work

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Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Cardiolipin (CL) is a lipid that is found in the membranes of bacteria and the inner membranes of mitochondria. CL can increase the activity of integral membrane proteins, in particular components of respiratory pathways. We here report that CL activated detergent-solubilized cytochrome bd, a terminal oxidase from Escherichia coli. CL enhanced the oxygen consumption activity ~ twofold and decreased the apparent K_M value for ubiquinol-1 as substrate from 95 µM to 35 µM. Activation by CL was also observed for cytochrome bd from two Gram-positive species, Geobacillus thermodenitrificans and Corynebacterium glutamicum, and for cytochrome bo₃ from E. coli. Taken together, CL can enhance the activity of detergent-solubilized cytochrome bd and cytochrome bo₃.

Original language	English
Article number	8006
Pages (from-to)	1-8
Number of pages	8
Journal	Scientific Reports
Volume	11
Early online date	13 Apr 2021
DOIs	https://doi.org/10.1038/s41598-021-87354-0
Publication status	Published - Dec 2021

Bibliographical note

Funding Information:
This work was partly supported by a Grant-in-Aid for Scientific Research (C) (16K07299 to J.S.) from the Japan Society for the Promotion of Science.

Publisher Copyright:
© 2021, The Author(s).

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

Funding

This work was partly supported by a Grant-in-Aid for Scientific Research (C) (16K07299 to J.S.) from the Japan Society for the Promotion of Science.

Funders	Funder number
Japan Society for the Promotion of Science	16K07299
Japan Society for the Promotion of Science

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Cite this

@article{022ca24a299e4d6eb0b29a64b1a7d9da,

title = "Cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo 3 solubilized in dodecyl-maltoside",

abstract = "Cardiolipin (CL) is a lipid that is found in the membranes of bacteria and the inner membranes of mitochondria. CL can increase the activity of integral membrane proteins, in particular components of respiratory pathways. We here report that CL activated detergent-solubilized cytochrome bd, a terminal oxidase from Escherichia coli. CL enhanced the oxygen consumption activity ~ twofold and decreased the apparent KM value for ubiquinol-1 as substrate from 95 µM to 35 µM. Activation by CL was also observed for cytochrome bd from two Gram-positive species, Geobacillus thermodenitrificans and Corynebacterium glutamicum, and for cytochrome bo3 from E. coli. Taken together, CL can enhance the activity of detergent-solubilized cytochrome bd and cytochrome bo3.",

author = "Asseri, {Amer H.} and Albert Godoy-Hernandez and Goojani, {Hojjat Ghasemi} and Holger Lill and Junshi Sakamoto and McMillan, {Duncan G.G.} and Dirk Bald",

note = "Funding Information: This work was partly supported by a Grant-in-Aid for Scientific Research (C) (16K07299 to J.S.) from the Japan Society for the Promotion of Science. Publisher Copyright: {\textcopyright} 2021, The Author(s). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

year = "2021",

month = dec,

doi = "10.1038/s41598-021-87354-0",

language = "English",

volume = "11",

pages = "1--8",

journal = "Scientific Reports",

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T1 - Cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo 3 solubilized in dodecyl-maltoside

AU - Asseri, Amer H.

AU - Godoy-Hernandez, Albert

AU - Goojani, Hojjat Ghasemi

AU - Lill, Holger

AU - Sakamoto, Junshi

AU - McMillan, Duncan G.G.

AU - Bald, Dirk

N1 - Funding Information: This work was partly supported by a Grant-in-Aid for Scientific Research (C) (16K07299 to J.S.) from the Japan Society for the Promotion of Science. Publisher Copyright: © 2021, The Author(s). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/12

Y1 - 2021/12

N2 - Cardiolipin (CL) is a lipid that is found in the membranes of bacteria and the inner membranes of mitochondria. CL can increase the activity of integral membrane proteins, in particular components of respiratory pathways. We here report that CL activated detergent-solubilized cytochrome bd, a terminal oxidase from Escherichia coli. CL enhanced the oxygen consumption activity ~ twofold and decreased the apparent KM value for ubiquinol-1 as substrate from 95 µM to 35 µM. Activation by CL was also observed for cytochrome bd from two Gram-positive species, Geobacillus thermodenitrificans and Corynebacterium glutamicum, and for cytochrome bo3 from E. coli. Taken together, CL can enhance the activity of detergent-solubilized cytochrome bd and cytochrome bo3.

AB - Cardiolipin (CL) is a lipid that is found in the membranes of bacteria and the inner membranes of mitochondria. CL can increase the activity of integral membrane proteins, in particular components of respiratory pathways. We here report that CL activated detergent-solubilized cytochrome bd, a terminal oxidase from Escherichia coli. CL enhanced the oxygen consumption activity ~ twofold and decreased the apparent KM value for ubiquinol-1 as substrate from 95 µM to 35 µM. Activation by CL was also observed for cytochrome bd from two Gram-positive species, Geobacillus thermodenitrificans and Corynebacterium glutamicum, and for cytochrome bo3 from E. coli. Taken together, CL can enhance the activity of detergent-solubilized cytochrome bd and cytochrome bo3.

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