Abstract
This chapter aims to explore various parameters involved in achieving high-end capillary electrophoresis hyphenated to mass spectrometry (CE-MS) analysis of proteins, peptides, and their posttranslational modifications. The structure of the topics discussed in this book chapter is conveniently mapped on the scheme of the CE-MS system itself, starting from sample preconcentration and injection techniques and finishing with mass analyzer considerations. After going through the technical considerations, a variety of relevant applications for this analytical approach are presented, including posttranslational modifications analysis, clinical biomarker discovery, and its growing use in the biotechnological industry.
Original language | English |
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Title of host publication | Separation Techniques Applied to Omics Sciences |
Subtitle of host publication | From Principles to Relevant Applications |
Editors | Ana Valéria Colnaghi Simionato |
Publisher | Springer |
Pages | 51-86 |
Number of pages | 36 |
ISBN (Electronic) | 9783030772529 |
ISBN (Print) | 9783030772512, 9783030772543 |
DOIs | |
Publication status | Published - 2021 |
Publication series
Name | Advances in Experimental Medicine and Biology |
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Volume | 1336 |
ISSN (Print) | 0065-2598 |
ISSN (Electronic) | 2214-8019 |
Bibliographical note
Publisher Copyright:© 2021, Springer Nature Switzerland AG.
Keywords
- Biomarker discovery
- Biopharmaceuticals analysis
- CE separation optimization
- CE-MS interfacing
- CE-MS, Proteomics
- Intact protein analysis
- MS fragmentation
- PTM analysis
- Sample concentration techniques