CE-MS for Proteomics and Intact Protein Analysis

Valeriia O. Kuzyk, Govert W. Somsen, Rob Haselberg*

*Corresponding author for this work

Research output: Chapter in Book / Report / Conference proceedingChapterAcademicpeer-review

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Abstract

This chapter aims to explore various parameters involved in achieving high-end capillary electrophoresis hyphenated to mass spectrometry (CE-MS) analysis of proteins, peptides, and their posttranslational modifications. The structure of the topics discussed in this book chapter is conveniently mapped on the scheme of the CE-MS system itself, starting from sample preconcentration and injection techniques and finishing with mass analyzer considerations. After going through the technical considerations, a variety of relevant applications for this analytical approach are presented, including posttranslational modifications analysis, clinical biomarker discovery, and its growing use in the biotechnological industry.

Original languageEnglish
Title of host publicationSeparation Techniques Applied to Omics Sciences
Subtitle of host publicationFrom Principles to Relevant Applications
EditorsAna Valéria Colnaghi Simionato
PublisherSpringer
Pages51-86
Number of pages36
ISBN (Electronic)9783030772529
ISBN (Print)9783030772512, 9783030772543
DOIs
Publication statusPublished - 2021

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1336
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Bibliographical note

Publisher Copyright:
© 2021, Springer Nature Switzerland AG.

Keywords

  • Biomarker discovery
  • Biopharmaceuticals analysis
  • CE separation optimization
  • CE-MS interfacing
  • CE-MS, Proteomics
  • Intact protein analysis
  • MS fragmentation
  • PTM analysis
  • Sample concentration techniques

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