TY - JOUR
T1 - Changed isoflavone levels in red clover (Trifolium pratense L.) leaves with disturbed root nodulation in response to waterlogging.
AU - de Rijke, E.
AU - Aardenburg, L.
AU - van Dijk, J.
AU - Ariese, F.
AU - Ernst, W.H.O.
AU - Gooijer, C.
AU - Brinkman, U.A.T.
PY - 2005
Y1 - 2005
N2 - The effect of disturbed root nodulation on the quantitative and qualitative composition of the main isoflavonoid glucoside-malonates, glucosides, and aglycones in the leaves of Trifolium pratense L. grown under waterlogging conditions was investigated. Isoflavonoids are involved in the regulation of root nodule activity and the establishment of the mycorrhizal association. Isoflavonoid determination was performed using reversed-phase liquid chromatography coupled to mass spectrometric and UV absorbance detection. In response to waterlogging, the concentrations of biochanin A and biochanin A-7-O-glucoside-malonate, biochanin A-7-O-glucoside, and genistein-7-O-glucoside in the leaves increased two- to threefold after a lag period of 3 wk because of disturbed root nodulation. The other isoflavones detected-formononetin, formononetin-7-O-glucoside-malonate, and formononetin-7-O-glucoside-did not show any significant changes related to waterlogging. After restoring normal soil water conditions, the concentrations of biochanin A and its glucoside and glucoside-malonate rapidly returned to the initial values, whereas the concentration of genistein-7-O-glucoside remained high. © 2005 Springer Science + Business Media, Inc.
AB - The effect of disturbed root nodulation on the quantitative and qualitative composition of the main isoflavonoid glucoside-malonates, glucosides, and aglycones in the leaves of Trifolium pratense L. grown under waterlogging conditions was investigated. Isoflavonoids are involved in the regulation of root nodule activity and the establishment of the mycorrhizal association. Isoflavonoid determination was performed using reversed-phase liquid chromatography coupled to mass spectrometric and UV absorbance detection. In response to waterlogging, the concentrations of biochanin A and biochanin A-7-O-glucoside-malonate, biochanin A-7-O-glucoside, and genistein-7-O-glucoside in the leaves increased two- to threefold after a lag period of 3 wk because of disturbed root nodulation. The other isoflavones detected-formononetin, formononetin-7-O-glucoside-malonate, and formononetin-7-O-glucoside-did not show any significant changes related to waterlogging. After restoring normal soil water conditions, the concentrations of biochanin A and its glucoside and glucoside-malonate rapidly returned to the initial values, whereas the concentration of genistein-7-O-glucoside remained high. © 2005 Springer Science + Business Media, Inc.
U2 - 10.1007/s10886-005-5286-1
DO - 10.1007/s10886-005-5286-1
M3 - Article
SN - 0098-0331
VL - 31
SP - 1285
EP - 1298
JO - Journal of Chemical Ecology
JF - Journal of Chemical Ecology
ER -