TY - JOUR
T1 - Characterization of lipids in complex samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry
AU - Jover, E.
AU - Adahchour, M.
AU - Bayona, J.M.
AU - Vreuls, R.J.J.
AU - Brinkman, U.A.T.
PY - 2005
Y1 - 2005
N2 - Most lipids are a complex mixture of classes of compounds such as fatty acids, fatty alcohols, diols, sterols and hydroxy acids. In this study, the suitability of comprehensive two-dimensional gas chromatography coupled to a time-of-light mass spectrometer is studied for lipid characterization in complex samples. With lanolin, a refined wool wax, as test sample, it is demonstrated that combined methylation plus silylation is the preferred derivatization procedure to achieve (i) high-quality GC × GC separation and (ii) easily recognizable ordered structures in lipid analysis. Optimization of the GC × GC column combination, the influence of the temperature programme on the quality of the separation, and the potential and limitations of automated TOF-MS-based identification are discussed. The combined power of a 2D separation, ordered structures and MS detection is illustrated by the identification of several minor sample constituents. © 2005 Elsevier B.V. All rights reserved.
AB - Most lipids are a complex mixture of classes of compounds such as fatty acids, fatty alcohols, diols, sterols and hydroxy acids. In this study, the suitability of comprehensive two-dimensional gas chromatography coupled to a time-of-light mass spectrometer is studied for lipid characterization in complex samples. With lanolin, a refined wool wax, as test sample, it is demonstrated that combined methylation plus silylation is the preferred derivatization procedure to achieve (i) high-quality GC × GC separation and (ii) easily recognizable ordered structures in lipid analysis. Optimization of the GC × GC column combination, the influence of the temperature programme on the quality of the separation, and the potential and limitations of automated TOF-MS-based identification are discussed. The combined power of a 2D separation, ordered structures and MS detection is illustrated by the identification of several minor sample constituents. © 2005 Elsevier B.V. All rights reserved.
U2 - 10.1016/j.chroma.2005.05.093
DO - 10.1016/j.chroma.2005.05.093
M3 - Article
SN - 0021-9673
VL - 1086
SP - 2
EP - 11
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -
