Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

Josefine Hirschfeld; Ilaria J. Chicca; Carolyn G.J. Moonen; Phillipa C. White; Martin R. Ling; Helen J. Wright; Paul R. Cooper; Mike R. Milward; Iain L.C. Chapple

doi:10.1007/978-1-0716-2780-8_27

Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

Josefine Hirschfeld^*, Ilaria J. Chicca, Carolyn G.J. Moonen, Phillipa C. White, Martin R. Ling, Helen J. Wright, Paul R. Cooper, Mike R. Milward, Iain L.C. Chapple

^*Corresponding author for this work

Periodontology

Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review

Abstract

Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils as well as of oral neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.

Original language	English
Title of host publication	Oral Biology
Subtitle of host publication	Molecular Techniques and Applications
Editors	Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng, Paul R. Cooper
Publisher	Humana Press Inc
Pages	451-472
Number of pages	22
ISBN (Electronic)	9781071627808
ISBN (Print)	9781071627792, 9781071627822
DOIs	https://doi.org/10.1007/978-1-0716-2780-8_27
Publication status	Published - 2023

Publication series

Name	Methods in Molecular Biology
Volume	2588
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

The original version of this chapter was revised. The correction to this chapter is available at https://doi.org/10.1007/978-1-0716-2780-8_32

Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Cathepsin G
Chemiluminescence
DNA
Elastase
Fluorescence microscopy
Immunostaining
Myeloperoxidase
Neutrophil extracellular traps
Reactive oxygen species
SEM

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-0716-2780-8_27

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Hirschfeld, J., Chicca, I. J., Moonen, C. G. J., White, P. C., Ling, M. R., Wright, H. J., Cooper, P. R., Milward, M. R., & Chapple, I. L. C. (2023). Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps. In G. J. Seymour, M. P. Cullinan, N. C. K. Heng, & P. R. Cooper (Eds.), Oral Biology: Molecular Techniques and Applications (pp. 451-472). (Methods in Molecular Biology; Vol. 2588). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2780-8_27

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abstract = "Following the discovery of neutrophil extracellular traps (NETs) in 2004 by Brinkmann and colleagues, there has been extensive research into the role of NETs in a number of inflammatory diseases, including periodontitis. This chapter describes the current methods for the isolation of peripheral blood neutrophils as well as of oral neutrophils for subsequent NET experiments, including approaches to quantify and visualize NET production, the ability of NETs to entrap and kill bacteria, and the removal of NETs by nuclease-containing plasma.",

keywords = "Cathepsin G, Chemiluminescence, DNA, Elastase, Fluorescence microscopy, Immunostaining, Myeloperoxidase, Neutrophil extracellular traps, Reactive oxygen species, SEM",

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note = "The original version of this chapter was revised. The correction to this chapter is available at https://doi.org/10.1007/978-1-0716-2780-8_32 Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

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Hirschfeld, J, Chicca, IJ, Moonen, CGJ, White, PC, Ling, MR, Wright, HJ, Cooper, PR, Milward, MR & Chapple, ILC 2023, Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps. in GJ Seymour, MP Cullinan, NCK Heng & PR Cooper (eds), Oral Biology: Molecular Techniques and Applications. Methods in Molecular Biology, vol. 2588, Humana Press Inc, pp. 451-472. https://doi.org/10.1007/978-1-0716-2780-8_27

Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps. / Hirschfeld, Josefine; Chicca, Ilaria J.; Moonen, Carolyn G.J. et al.
Oral Biology: Molecular Techniques and Applications. ed. / Gregory J. Seymour; Mary P. Cullinan; Nicholas C.K. Heng; Paul R. Cooper. Humana Press Inc, 2023. p. 451-472 (Methods in Molecular Biology; Vol. 2588).

Research output: Chapter in Book / Report / Conference proceeding › Chapter › Academic › peer-review

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AU - Chicca, Ilaria J.

AU - Moonen, Carolyn G.J.

AU - White, Phillipa C.

AU - Ling, Martin R.

AU - Wright, Helen J.

AU - Cooper, Paul R.

AU - Milward, Mike R.

AU - Chapple, Iain L.C.

N1 - The original version of this chapter was revised. The correction to this chapter is available at https://doi.org/10.1007/978-1-0716-2780-8_32 Publisher Copyright: © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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KW - Cathepsin G

KW - Chemiluminescence

KW - DNA

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KW - Fluorescence microscopy

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PB - Humana Press Inc

ER -

Hirschfeld J, Chicca IJ, Moonen CGJ, White PC, Ling MR, Wright HJ et al. Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps. In Seymour GJ, Cullinan MP, Heng NCK, Cooper PR, editors, Oral Biology: Molecular Techniques and Applications. Humana Press Inc. 2023. p. 451-472. (Methods in Molecular Biology). Epub 2022 Nov 23. doi: 10.1007/978-1-0716-2780-8_27

Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

Abstract

Publication series

Bibliographical note

Keywords

UN SDGs

Access to Document

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