TY - JOUR
T1 - Charge transfer states in phycobilisomes
AU - Wahadoszamen, Md
AU - Krüger, Tjaart P.J.
AU - Ara, Anjue Mane
AU - van Grondelle, Rienk
AU - Gwizdala, Michal
PY - 2020/7/1
Y1 - 2020/7/1
N2 - Phycobilisomes (PBs) absorb light and supply downstream photosynthetic processes with excitation energy in many cyanobacteria and algae. In response to a sudden increase in light intensity, excess excitation energy is photoprotectively dissipated in PBs by means of the orange carotenoid protein (OCP)-related mechanism or via a light-activated intrinsic decay channel. Recently, we have identified that both mechanisms are associated with far-red emission states. Here, we investigate the far-red states involved with the light-induced intrinsic mechanism by exploring the energy landscape and electro-optical properties of the pigments in PBs. While Stark spectroscopy showed that the far-red states in PBs exhibit a strong charge-transfer (CT) character at cryogenic temperatures, single molecule spectroscopy revealed that CT states should also be present at room temperature. Owing to the strong environmental sensitivity of CT states, the knowledge gained from this study may contribute to the design of a new generation of fluorescence markers.
AB - Phycobilisomes (PBs) absorb light and supply downstream photosynthetic processes with excitation energy in many cyanobacteria and algae. In response to a sudden increase in light intensity, excess excitation energy is photoprotectively dissipated in PBs by means of the orange carotenoid protein (OCP)-related mechanism or via a light-activated intrinsic decay channel. Recently, we have identified that both mechanisms are associated with far-red emission states. Here, we investigate the far-red states involved with the light-induced intrinsic mechanism by exploring the energy landscape and electro-optical properties of the pigments in PBs. While Stark spectroscopy showed that the far-red states in PBs exhibit a strong charge-transfer (CT) character at cryogenic temperatures, single molecule spectroscopy revealed that CT states should also be present at room temperature. Owing to the strong environmental sensitivity of CT states, the knowledge gained from this study may contribute to the design of a new generation of fluorescence markers.
KW - Excitation energy flow regulation
KW - Light harvesting
KW - Photosynthesis
KW - Single molecule spectroscopy
KW - Stark spectroscopy
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U2 - 10.1016/j.bbabio.2020.148187
DO - 10.1016/j.bbabio.2020.148187
M3 - Article
C2 - 32173383
AN - SCOPUS:85082132204
VL - 1861
SP - 1
EP - 10
JO - Biochimica et Biophysica Acta (BBA) - Bioenergetics
JF - Biochimica et Biophysica Acta (BBA) - Bioenergetics
SN - 0005-2728
IS - 7
M1 - 148187
ER -