Charge transfer states in phycobilisomes

Md Wahadoszamen; Tjaart P.J. Krüger; Anjue Mane Ara; Rienk van Grondelle; Michal Gwizdala

doi:10.1016/j.bbabio.2020.148187

Charge transfer states in phycobilisomes

Md Wahadoszamen, Tjaart P.J. Krüger, Anjue Mane Ara, Rienk van Grondelle, Michal Gwizdala^*

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

149 Downloads (Pure)

Abstract

Phycobilisomes (PBs) absorb light and supply downstream photosynthetic processes with excitation energy in many cyanobacteria and algae. In response to a sudden increase in light intensity, excess excitation energy is photoprotectively dissipated in PBs by means of the orange carotenoid protein (OCP)-related mechanism or via a light-activated intrinsic decay channel. Recently, we have identified that both mechanisms are associated with far-red emission states. Here, we investigate the far-red states involved with the light-induced intrinsic mechanism by exploring the energy landscape and electro-optical properties of the pigments in PBs. While Stark spectroscopy showed that the far-red states in PBs exhibit a strong charge-transfer (CT) character at cryogenic temperatures, single molecule spectroscopy revealed that CT states should also be present at room temperature. Owing to the strong environmental sensitivity of CT states, the knowledge gained from this study may contribute to the design of a new generation of fluorescence markers.

Original language	English
Article number	148187
Pages (from-to)	1-10
Number of pages	10
Journal	Biochimica et Biophysica Acta - Bioenergetics
Volume	1861
Issue number	7
Early online date	12 Mar 2020
DOIs	https://doi.org/10.1016/j.bbabio.2020.148187
Publication status	Published - 1 Jul 2020

Funding

We would like to acknowledge Dr. Diana Kirilovsky for kindly letting us use her laboratory to obtain the samples used in this study. We thank Dr. Ghada Ajlani for the kind gift of the mutant strains of Synechocystis , from which the samples were isolated. The work of all authors was supported from an advanced investigator grant ( 267333 , PHOTPROT) from the European Research Council and the TOP grant ( 700.58.305 ) from the Foundation of Chemical Sciences part of NWO , both awarded to RvG. TPJK was supported by the National Equipment Programme of the National Research Foundation (NRF) (grant N00500 , project 87990), NRF Thuthuka programme (grant N00726 , project 94107) and the Photonics Initiative of South Africa . RvG gratefully acknowledges his ‘Academy Professor’ grant from the Royal Netherlands Academy of Arts and Sciences (KNAW) to RvG EMBO long term fellowship, Claude Leon Foundation Postdoctoral Fellowship and Senior Postdoctoral fellowship at the University of Pretoria to MG.

Funders	Funder number
PHOTPROT
Seventh Framework Programme	267333
European Research Council	700.58.305
National Research Foundation	N00500, 94107, N00726, 87990
Claude Leon Foundation
University of Pretoria
Koninklijke Nederlandse Akademie van Wetenschappen
Nederlandse Organisatie voor Wetenschappelijk Onderzoek

Keywords

Excitation energy flow regulation
Light harvesting
Photosynthesis
Single molecule spectroscopy
Stark spectroscopy

Access to Document

10.1016/j.bbabio.2020.148187

Charge transfer states in phycobilisomesFinal published version, 1.36 MBLicence: Article 25fa Dutch Copyright Act

Persistent URL (handle)

Persistent link

Cite this

@article{400f1c8ee30740fc9d36a874bdc433e9,

title = "Charge transfer states in phycobilisomes",

abstract = "Phycobilisomes (PBs) absorb light and supply downstream photosynthetic processes with excitation energy in many cyanobacteria and algae. In response to a sudden increase in light intensity, excess excitation energy is photoprotectively dissipated in PBs by means of the orange carotenoid protein (OCP)-related mechanism or via a light-activated intrinsic decay channel. Recently, we have identified that both mechanisms are associated with far-red emission states. Here, we investigate the far-red states involved with the light-induced intrinsic mechanism by exploring the energy landscape and electro-optical properties of the pigments in PBs. While Stark spectroscopy showed that the far-red states in PBs exhibit a strong charge-transfer (CT) character at cryogenic temperatures, single molecule spectroscopy revealed that CT states should also be present at room temperature. Owing to the strong environmental sensitivity of CT states, the knowledge gained from this study may contribute to the design of a new generation of fluorescence markers.",

keywords = "Excitation energy flow regulation, Light harvesting, Photosynthesis, Single molecule spectroscopy, Stark spectroscopy",

author = "Md Wahadoszamen and Kr{\"u}ger, {Tjaart P.J.} and Ara, {Anjue Mane} and {van Grondelle}, Rienk and Michal Gwizdala",

year = "2020",

month = jul,

day = "1",

doi = "10.1016/j.bbabio.2020.148187",

language = "English",

volume = "1861",

pages = "1--10",

journal = "Biochimica et Biophysica Acta - Bioenergetics",

issn = "0005-2728",

publisher = "Elsevier B.V.",

number = "7",

}

TY - JOUR

T1 - Charge transfer states in phycobilisomes

AU - Wahadoszamen, Md

AU - Krüger, Tjaart P.J.

AU - Ara, Anjue Mane

AU - van Grondelle, Rienk

AU - Gwizdala, Michal

PY - 2020/7/1

Y1 - 2020/7/1

N2 - Phycobilisomes (PBs) absorb light and supply downstream photosynthetic processes with excitation energy in many cyanobacteria and algae. In response to a sudden increase in light intensity, excess excitation energy is photoprotectively dissipated in PBs by means of the orange carotenoid protein (OCP)-related mechanism or via a light-activated intrinsic decay channel. Recently, we have identified that both mechanisms are associated with far-red emission states. Here, we investigate the far-red states involved with the light-induced intrinsic mechanism by exploring the energy landscape and electro-optical properties of the pigments in PBs. While Stark spectroscopy showed that the far-red states in PBs exhibit a strong charge-transfer (CT) character at cryogenic temperatures, single molecule spectroscopy revealed that CT states should also be present at room temperature. Owing to the strong environmental sensitivity of CT states, the knowledge gained from this study may contribute to the design of a new generation of fluorescence markers.

AB - Phycobilisomes (PBs) absorb light and supply downstream photosynthetic processes with excitation energy in many cyanobacteria and algae. In response to a sudden increase in light intensity, excess excitation energy is photoprotectively dissipated in PBs by means of the orange carotenoid protein (OCP)-related mechanism or via a light-activated intrinsic decay channel. Recently, we have identified that both mechanisms are associated with far-red emission states. Here, we investigate the far-red states involved with the light-induced intrinsic mechanism by exploring the energy landscape and electro-optical properties of the pigments in PBs. While Stark spectroscopy showed that the far-red states in PBs exhibit a strong charge-transfer (CT) character at cryogenic temperatures, single molecule spectroscopy revealed that CT states should also be present at room temperature. Owing to the strong environmental sensitivity of CT states, the knowledge gained from this study may contribute to the design of a new generation of fluorescence markers.

KW - Excitation energy flow regulation

KW - Light harvesting

KW - Photosynthesis

KW - Single molecule spectroscopy

KW - Stark spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=85082132204&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85082132204&partnerID=8YFLogxK

U2 - 10.1016/j.bbabio.2020.148187

DO - 10.1016/j.bbabio.2020.148187

M3 - Article

C2 - 32173383

AN - SCOPUS:85082132204

SN - 0005-2728

VL - 1861

SP - 1

EP - 10

JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

IS - 7

M1 - 148187

ER -

Charge transfer states in phycobilisomes

Abstract

Funding

Keywords

Access to Document

Persistent URL (handle)

Other files and links

Fingerprint

Cite this