Chromatography-mass spectrometry based analysis of biologically active endogenous carboxylic acids

D. Kloos, H. Lingeman, O.A. Mayboroda, A.M. Deelder, W.M.A. Niessen, M. Giera

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Carboxylic acids (CAs), such as small CAs, fatty acids, eicosanoids and bile acids, are involved in numerous biological processes. Mapping the participating pathways potentially yields great insight into the physiological state of an organism. There is demand for analytical techniques that are more sensitive, faster and easier to handle. These would enable advanced analysis and validation in larger clinical cohorts and hopefully deliver useful disease markers. The analysis of CAs is not only driven by the CA group, but also largely dependent on chain length and other functional groups present. Several liquid and gas chromatography strategies, with or without derivatization, were developed in recent years. Here, we review the most recent trends in analysis of endogenous CAs by chromatography-mass spectrometry. We critically evaluate sample preparation, derivatization techniques, separation and mass spectrometric detection. Ultimately, the reader is guided to the most versatile, sensitive and easy analytical methods.
Original languageEnglish
Pages (from-to)17-28
JournalTrAC. Trends in Analytical Chemistry
Volume61
DOIs
Publication statusPublished - 2014

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Carboxylic Acids
Chromatography
Mass spectrometry
Eicosanoids
Liquid chromatography
Bile Acids and Salts
Chain length
Gas chromatography
Functional groups
Fatty Acids

Cite this

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title = "Chromatography-mass spectrometry based analysis of biologically active endogenous carboxylic acids",
abstract = "Carboxylic acids (CAs), such as small CAs, fatty acids, eicosanoids and bile acids, are involved in numerous biological processes. Mapping the participating pathways potentially yields great insight into the physiological state of an organism. There is demand for analytical techniques that are more sensitive, faster and easier to handle. These would enable advanced analysis and validation in larger clinical cohorts and hopefully deliver useful disease markers. The analysis of CAs is not only driven by the CA group, but also largely dependent on chain length and other functional groups present. Several liquid and gas chromatography strategies, with or without derivatization, were developed in recent years. Here, we review the most recent trends in analysis of endogenous CAs by chromatography-mass spectrometry. We critically evaluate sample preparation, derivatization techniques, separation and mass spectrometric detection. Ultimately, the reader is guided to the most versatile, sensitive and easy analytical methods.",
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Chromatography-mass spectrometry based analysis of biologically active endogenous carboxylic acids. / Kloos, D.; Lingeman, H.; Mayboroda, O.A.; Deelder, A.M.; Niessen, W.M.A.; Giera, M.

In: TrAC. Trends in Analytical Chemistry, Vol. 61, 2014, p. 17-28.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Chromatography-mass spectrometry based analysis of biologically active endogenous carboxylic acids

AU - Kloos, D.

AU - Lingeman, H.

AU - Mayboroda, O.A.

AU - Deelder, A.M.

AU - Niessen, W.M.A.

AU - Giera, M.

PY - 2014

Y1 - 2014

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AB - Carboxylic acids (CAs), such as small CAs, fatty acids, eicosanoids and bile acids, are involved in numerous biological processes. Mapping the participating pathways potentially yields great insight into the physiological state of an organism. There is demand for analytical techniques that are more sensitive, faster and easier to handle. These would enable advanced analysis and validation in larger clinical cohorts and hopefully deliver useful disease markers. The analysis of CAs is not only driven by the CA group, but also largely dependent on chain length and other functional groups present. Several liquid and gas chromatography strategies, with or without derivatization, were developed in recent years. Here, we review the most recent trends in analysis of endogenous CAs by chromatography-mass spectrometry. We critically evaluate sample preparation, derivatization techniques, separation and mass spectrometric detection. Ultimately, the reader is guided to the most versatile, sensitive and easy analytical methods.

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DO - 10.1016/j.trac.2014.05.008

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JO - TrAC. Trends in Analytical Chemistry

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SN - 0165-9936

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