Circumventing the Crabtree effect in cell culture: A systematic review

Michèle J.C. de Kok; Alexander F. Schaapherder; Rob C.I. Wüst; Melissa Zuiderwijk; Jaap A. Bakker; Jan H.N. Lindeman; Sylvia E. Le Dévédec

doi:10.1016/j.mito.2021.03.014

Circumventing the Crabtree effect in cell culture: A systematic review

Michèle J.C. de Kok, Alexander F. Schaapherder, Rob C.I. Wüst, Melissa Zuiderwijk, Jaap A. Bakker, Jan H.N. Lindeman, Sylvia E. Le Dévédec^*

^*Corresponding author for this work

Research output: Contribution to Journal › Review article › Academic › peer-review

Abstract

Metabolic reprogramming and mitochondrial dysfunction are central elements in a broad variety of physiological and pathological processes. While cell culture established itself as a versatile technique for the elaboration of physiology and disease, studying metabolism using standard cell culture protocols is profoundly interfered by the Crabtree effect. This phenomenon refers to the adaptation of cultured cells to a glycolytic phenotype, away from oxidative phosphorylation in glucose-containing medium, and questions the applicability of cell culture in certain fields of research. In this systematic review we aim to provide a comprehensive overview and critical appraisal of strategies reported to circumvent the Crabtree effect.

Original language	English
Pages (from-to)	83-95
Number of pages	13
Journal	Mitochondrion
Volume	59
Early online date	1 Apr 2021
DOIs	https://doi.org/10.1016/j.mito.2021.03.014
Publication status	Published - Jul 2021

Bibliographical note

Funding Information:
This work was supported by the Dutch Kidney Foundation (Metabolic salvage strategies to improve transplant outcome. Project 17O/11.)

Publisher Copyright:
© 2021 The Authors

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

Funding

This work was supported by the Dutch Kidney Foundation (Metabolic salvage strategies to improve transplant outcome. Project 17O/11.)

Funders	Funder number
Nierstichting	17O/11
Leids Universitair Medisch Centrum

Keywords

Cell culture
Crabtree effect
Glycolysis
Metabolism
Mitochondria
Oxidative phosphorylation

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10.1016/j.mito.2021.03.014

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title = "Circumventing the Crabtree effect in cell culture: A systematic review",

abstract = "Metabolic reprogramming and mitochondrial dysfunction are central elements in a broad variety of physiological and pathological processes. While cell culture established itself as a versatile technique for the elaboration of physiology and disease, studying metabolism using standard cell culture protocols is profoundly interfered by the Crabtree effect. This phenomenon refers to the adaptation of cultured cells to a glycolytic phenotype, away from oxidative phosphorylation in glucose-containing medium, and questions the applicability of cell culture in certain fields of research. In this systematic review we aim to provide a comprehensive overview and critical appraisal of strategies reported to circumvent the Crabtree effect.",

keywords = "Cell culture, Crabtree effect, Glycolysis, Metabolism, Mitochondria, Oxidative phosphorylation",

author = "{de Kok}, {Mich{\`e}le J.C.} and Schaapherder, {Alexander F.} and W{\"u}st, {Rob C.I.} and Melissa Zuiderwijk and Bakker, {Jaap A.} and Lindeman, {Jan H.N.} and {Le D{\'e}v{\'e}dec}, {Sylvia E.}",

note = "Funding Information: This work was supported by the Dutch Kidney Foundation (Metabolic salvage strategies to improve transplant outcome. Project 17O/11.) Publisher Copyright: {\textcopyright} 2021 The Authors Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

year = "2021",

month = jul,

doi = "10.1016/j.mito.2021.03.014",

language = "English",

volume = "59",

pages = "83--95",

journal = "Mitochondrion",

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T2 - A systematic review

AU - de Kok, Michèle J.C.

AU - Schaapherder, Alexander F.

AU - Wüst, Rob C.I.

AU - Zuiderwijk, Melissa

AU - Bakker, Jaap A.

AU - Lindeman, Jan H.N.

AU - Le Dévédec, Sylvia E.

N1 - Funding Information: This work was supported by the Dutch Kidney Foundation (Metabolic salvage strategies to improve transplant outcome. Project 17O/11.) Publisher Copyright: © 2021 The Authors Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/7

Y1 - 2021/7

N2 - Metabolic reprogramming and mitochondrial dysfunction are central elements in a broad variety of physiological and pathological processes. While cell culture established itself as a versatile technique for the elaboration of physiology and disease, studying metabolism using standard cell culture protocols is profoundly interfered by the Crabtree effect. This phenomenon refers to the adaptation of cultured cells to a glycolytic phenotype, away from oxidative phosphorylation in glucose-containing medium, and questions the applicability of cell culture in certain fields of research. In this systematic review we aim to provide a comprehensive overview and critical appraisal of strategies reported to circumvent the Crabtree effect.

AB - Metabolic reprogramming and mitochondrial dysfunction are central elements in a broad variety of physiological and pathological processes. While cell culture established itself as a versatile technique for the elaboration of physiology and disease, studying metabolism using standard cell culture protocols is profoundly interfered by the Crabtree effect. This phenomenon refers to the adaptation of cultured cells to a glycolytic phenotype, away from oxidative phosphorylation in glucose-containing medium, and questions the applicability of cell culture in certain fields of research. In this systematic review we aim to provide a comprehensive overview and critical appraisal of strategies reported to circumvent the Crabtree effect.

KW - Cell culture

KW - Crabtree effect

KW - Glycolysis

KW - Metabolism

KW - Mitochondria

KW - Oxidative phosphorylation

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DO - 10.1016/j.mito.2021.03.014

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SN - 1567-7249

VL - 59

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EP - 95

JO - Mitochondrion

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ER -

Circumventing the Crabtree effect in cell culture: A systematic review

Abstract

Bibliographical note

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Keywords

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