Metabolic reprogramming and mitochondrial dysfunction are central elements in a broad variety of physiological and pathological processes. While cell culture established itself as a versatile technique for the elaboration of physiology and disease, studying metabolism using standard cell culture protocols is profoundly interfered by the Crabtree effect. This phenomenon refers to the adaptation of cultured cells to a glycolytic phenotype, away from oxidative phosphorylation in glucose-containing medium, and questions the applicability of cell culture in certain fields of research. In this systematic review we aim to provide a comprehensive overview and critical appraisal of strategies reported to circumvent the Crabtree effect.
Bibliographical noteFunding Information:
This work was supported by the Dutch Kidney Foundation (Metabolic salvage strategies to improve transplant outcome. Project 17O/11.)
© 2021 The Authors
Copyright 2021 Elsevier B.V., All rights reserved.
- Cell culture
- Crabtree effect
- Oxidative phosphorylation