Climbing fiber input shapes reciprocity of purkinje cell firing

Aleksandra Badura, Martijn Schonewille, Kai Voges, Elisa Galliano, Nicolas Renier, Zhenyu Gao, Laurens Witter, Freek E. Hoebeek, Alain Chédotal, Chris I. DeZeeuw*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

The cerebellum fine-tunes motor activity via its Purkinje cell output. Purkinje cells produce two different types of spikes, complex spikes and simple spikes, which often show reciprocal activity:a periodical increase in complex spikes is associated with a decrease in simple spikes, and vice versa. This reciprocal firing is thought to be essential for coordinated motor behavior, yet how it is accomplished is debated. Here, we show in Ptf1a::cre;Robo3lox/lox mice that selectively rerouting the climbing fibers from a contralateral to an ipsilateral projection reversed the complex-spike modulation during sensory stimulation. Strikingly, modulation of simple spikes, which is supposed to be controlled by mossy fibers, reversed as well. Climbing fibers enforce this reciprocity in part by influencing activity of inhibitory interneurons, because the phase of their activity was also converted. Ptf1a::cre;Robo3lox/lox mice showed severe ataxia highlighting that climbing fiber input and its impact on reciprocity of Purkinje cell firing play an important role in motor coordination

Original languageEnglish
Pages (from-to)700-713
Number of pages14
JournalNeuron
Volume78
Issue number4
DOIs
Publication statusPublished - 22 May 2013
Externally publishedYes

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