Cloning and functional characterization of a testicular TSH receptor cDNA from the African catfish (Clarias gariepinus)

H F Vischer, J. Bogerd

Research output: Contribution to JournalArticleAcademicpeer-review


A cDNA encoding a putative thyroid-stimulating hormone receptor (cfTSH-R) was cloned from the testis of the African catfish (Clarias gariepinus). The cfTSH-R showed the highest amino acid sequence identity with the TSH-Rs of other fish species. In addition, an insertion of approximately 50 amino acids, specific for the TSH-R subfamily, was also present in the carboxy terminus of the amino-terminal extracellular domain of the cfTSH-R. Next to the testis and thyroid follicles, abundant cfTSH-R expression was detected in cerebellum, brain, ovary, seminal vesicles and pituitary, while weaker expression was found in muscle, stomach, intestine, head-kidney, liver, kidney and heart. HEK-T 293 cells, transiently expressing the cfTSH-R, significantly increased intracellular cAMP levels in response to human TSH. Catfish LH, human choriogonadotropin and human FSH were also able to induce this cfTSH-R-mediated response, although with considerably lower efficiency than human TSH. These results indicated that a functional cfTSH-R had been cloned from the testis of African catfish.

Original languageEnglish
Pages (from-to)227-38
Number of pages12
JournalJournal of molecular endocrinology
Issue number2
Publication statusPublished - Apr 2003


  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Catfishes
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary
  • Humans
  • Male
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Messenger
  • Receptors, Thyrotropin
  • Sequence Alignment
  • Testis
  • Tissue Distribution
  • Journal Article


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