Abstract
Mesenchymal stem cell and 3D printing-based bone tissue engineering present a promising technique to repair large-volume bone defects. Its success is highly dependent on cell attachment, spreading, osteogenic differentiation, and in vivo survival of stem cells on 3D-printed scaffolds. In this study, we applied human salivary histatin-1 (Hst1) to enhance the interactions of human adipose-derived stem cells (hASCs) on 3D-printed β-tricalcium phosphate (β-TCP) bioceramic scaffolds. Fluorescent images showed that Hst1 significantly enhanced the adhesion of hASCs to both bioinert glass and 3D-printed β-TCP scaffold. In addition, Hst1 was associated with significantly higher proliferation and osteogenic differentiation of hASCs on 3D-printed β-TCP scaffolds. Moreover, coating 3D-printed β-TCP scaffolds with histatin significantly promotes the survival of hASCs in vivo. The ERK and p38 but not JNK signaling was found to be involved in the superior adhesion of hASCs to β-TCP scaffolds with the aid of Hst1. In conclusion, Hst1 could significantly promote the adhesion, spreading, osteogenic differentiation, and in vivo survival of hASCs on 3D-printed β-TCP scaffolds, bearing a promising application in stem cell/3D printing-based constructs for bone tissue engineering.
| Original language | English |
|---|---|
| Pages (from-to) | 321-331 |
| Number of pages | 11 |
| Journal | Tissue Engineering. Part C, Methods |
| Volume | 29 |
| Issue number | 7 |
| Early online date | 18 Jul 2023 |
| DOIs | |
| Publication status | Published - Jul 2023 |
Funding
This research was supported by the Shenzhen Science and Technology Innovation Committee (JCYJ20210324105408022), High-End Foreign Expert Recruitment Plan of China (No. G20200216024), Key Research andDevelopment Plan of Zhejiang Province (No. 2021C04013), Department of Education of Guangdong Province (No. 2019KQNCX121), Guangzhou Municipal Health Commission (No. 20201A010067), and Guangzhou Medical University (No. 2020A046). This research was supported by the Shenzhen Science and Technology Innovation Committee (JCYJ20210324105408022), High-End Foreign Expert Recruitment Plan of China (No. G20200216024), Key Research and Development Plan of Zhejiang Province (No. 2021C04013), Department of Education of Guangdong Province (No. 2019KQNCX121), Guangzhou Municipal Health Commission (No. 20201A010067), and Guangzhou Medical University (No. 2020A046).
| Funders | Funder number |
|---|---|
| Guangzhou Municipal Health Commission | 20201A010067 |
| Plan of Zhejiang Province | |
| Guangzhou Medical University | 2020A046 |
| Key Research and Development Program of Zhejiang Province | 2021C04013 |
| Department of Education of Guangdong Province | 2019KQNCX121 |
| Science, Technology and Innovation Commission of Shenzhen Municipality | JCYJ20210324105408022 |
| High-end Foreign Experts Recruitment Plan of China | G20200216024 |
Keywords
- 3D printing
- adipose-derived stem cells
- bone tissue engineering
- histatin-1
- osteogenesis