Cold stress increases salt tolerance of the extremophytes Eutrema salsugineum (Thellungiella salsuginea) and Eutrema (Thellungiella) botschantzevii

A. O. Shamustakimova, G. Leonova, V. V. Taranov, A. H. de Boer, A. V. Babakov*

*Corresponding author for this work

Research output: Contribution to JournalArticleAcademicpeer-review


A comparative study was performed to analyze the effect of cold acclimation on improving the resistance of Arabidopsis thaliana, Eutrema salsugineum and Eutrema botschantzevii plants to salt stress. Shoot FW, sodium and potassium accumulation, metabolite content, expression of proton pump genes VAB1, VAB2,VAB3, VP2, HA3 and genes encoding ion transporters SOS1, HKT1, NHX1, NHX2, NHX5 located in the plasma membrane or tonoplast were determined just after the cold treatment and the onset of the salt stress. In the same cold-acclimated E. botschantzevii plants, the Na+ concentration after salt treatment was around 80% lower than in non-acclimated plants, whereas the K+ concentration was higher. As a result of cold acclimation, the expression of, VAB3, NHX2, NHX5 genes and of SOS1, VP2, HA3 genes was strongly enhanced in E. botschantzevii and in E. salsugineum plants correspondently. None of the 10 genes analyzed showed any expression change in A. thaliana plants after cold acclimation. Altogether, the results indicate that cold-induced adaptation to subsequent salt stress exists in the extremophytes E. botschantzevii and to a lesser extend in E. salsugineum and is absent in Arabidopsis. This phenomenon may be attributed to the increased expression of ion transporter genes during cold acclimation in the Eutrema species.

Original languageEnglish
Pages (from-to)128-138
Number of pages11
JournalJournal of Plant Physiology
Issue numberJanuary
Publication statusPublished - 1 Jan 2017


This work was supported by the Russian Foundation for Basic Research (grant 05-04-89005-NWО a, grant 14-04-00816 ) and by the Netherlands Organization for Scientific Research (NWO; grant 047.017.004 ). The work was performed with the use of scientific equipment of the Center for collective use ‘Biotechnology’ at the All-Russia Research Institute of Agricultural Biotechnology (Moscow, Russia; agreement RFMEFI62114X0003) Appendix A

FundersFunder number
Russian Foundation for Basic Research14-04-00816, 05-04-89005-NWО a
Nederlandse Organisatie voor Wetenschappelijk Onderzoek047.017.004


    • Arabidopsis thaliana
    • Cis-acting elements
    • Cold acclimation
    • Eutrema botschantzevii
    • Eutrema salsugineum
    • Gene expression
    • Ion transporter
    • K/Na accumulation
    • Metabolite content
    • Salt stress


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