Cold stress increases salt tolerance of the extremophytes Eutrema salsugineum (Thellungiella salsuginea) and Eutrema (Thellungiella) botschantzevii

A. O. Shamustakimova; G. Leonova; V. V. Taranov; A. H. de Boer; A. V. Babakov

doi:10.1016/j.jplph.2016.10.009

Cold stress increases salt tolerance of the extremophytes Eutrema salsugineum (Thellungiella salsuginea) and Eutrema (Thellungiella) botschantzevii

A. O. Shamustakimova
, G. Leonova
, V. V. Taranov
, A. H. de Boer
, A. V. Babakov^*

^*Corresponding author for this work

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

A comparative study was performed to analyze the effect of cold acclimation on improving the resistance of Arabidopsis thaliana, Eutrema salsugineum and Eutrema botschantzevii plants to salt stress. Shoot FW, sodium and potassium accumulation, metabolite content, expression of proton pump genes VAB1, VAB2,VAB3, VP2, HA3 and genes encoding ion transporters SOS1, HKT1, NHX1, NHX2, NHX5 located in the plasma membrane or tonoplast were determined just after the cold treatment and the onset of the salt stress. In the same cold-acclimated E. botschantzevii plants, the Na⁺ concentration after salt treatment was around 80% lower than in non-acclimated plants, whereas the K⁺ concentration was higher. As a result of cold acclimation, the expression of, VAB3, NHX2, NHX5 genes and of SOS1, VP2, HA3 genes was strongly enhanced in E. botschantzevii and in E. salsugineum plants correspondently. None of the 10 genes analyzed showed any expression change in A. thaliana plants after cold acclimation. Altogether, the results indicate that cold-induced adaptation to subsequent salt stress exists in the extremophytes E. botschantzevii and to a lesser extend in E. salsugineum and is absent in Arabidopsis. This phenomenon may be attributed to the increased expression of ion transporter genes during cold acclimation in the Eutrema species.

Original language	English
Pages (from-to)	128-138
Number of pages	11
Journal	Journal of Plant Physiology
Volume	208
Issue number	January
DOIs	https://doi.org/10.1016/j.jplph.2016.10.009
Publication status	Published - 1 Jan 2017

Funding

This work was supported by the Russian Foundation for Basic Research (grant 05-04-89005-NWО a, grant 14-04-00816 ) and by the Netherlands Organization for Scientific Research (NWO; grant 047.017.004 ). The work was performed with the use of scientific equipment of the Center for collective use ‘Biotechnology’ at the All-Russia Research Institute of Agricultural Biotechnology (Moscow, Russia; agreement RFMEFI62114X0003)

Funders	Funder number
Russian Foundation for Basic Research	14-04-00816, 05-04-89005-NWО a
Nederlandse Organisatie voor Wetenschappelijk Onderzoek	047.017.004

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 6 Clean Water and Sanitation

Keywords

Arabidopsis thaliana
Cis-acting elements
Cold acclimation
Eutrema botschantzevii
Eutrema salsugineum
Gene expression
Ion transporter
K/Na accumulation
Metabolite content
Salt stress

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10.1016/j.jplph.2016.10.009

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@article{54c9fe67f0f2474a946c4ff042e03d7c,

title = "Cold stress increases salt tolerance of the extremophytes Eutrema salsugineum (Thellungiella salsuginea) and Eutrema (Thellungiella) botschantzevii",

abstract = "A comparative study was performed to analyze the effect of cold acclimation on improving the resistance of Arabidopsis thaliana, Eutrema salsugineum and Eutrema botschantzevii plants to salt stress. Shoot FW, sodium and potassium accumulation, metabolite content, expression of proton pump genes VAB1, VAB2,VAB3, VP2, HA3 and genes encoding ion transporters SOS1, HKT1, NHX1, NHX2, NHX5 located in the plasma membrane or tonoplast were determined just after the cold treatment and the onset of the salt stress. In the same cold-acclimated E. botschantzevii plants, the Na+ concentration after salt treatment was around 80\% lower than in non-acclimated plants, whereas the K+ concentration was higher. As a result of cold acclimation, the expression of, VAB3, NHX2, NHX5 genes and of SOS1, VP2, HA3 genes was strongly enhanced in E. botschantzevii and in E. salsugineum plants correspondently. None of the 10 genes analyzed showed any expression change in A. thaliana plants after cold acclimation. Altogether, the results indicate that cold-induced adaptation to subsequent salt stress exists in the extremophytes E. botschantzevii and to a lesser extend in E. salsugineum and is absent in Arabidopsis. This phenomenon may be attributed to the increased expression of ion transporter genes during cold acclimation in the Eutrema species.",

keywords = "Arabidopsis thaliana, Cis-acting elements, Cold acclimation, Eutrema botschantzevii, Eutrema salsugineum, Gene expression, Ion transporter, K/Na accumulation, Metabolite content, Salt stress",

author = "Shamustakimova, \{A. O.\} and G. Leonova and Taranov, \{V. V.\} and \{de Boer\}, \{A. H.\} and Babakov, \{A. V.\}",

year = "2017",

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doi = "10.1016/j.jplph.2016.10.009",

language = "English",

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T1 - Cold stress increases salt tolerance of the extremophytes Eutrema salsugineum (Thellungiella salsuginea) and Eutrema (Thellungiella) botschantzevii

AU - Shamustakimova, A. O.

AU - Leonova, G.

AU - Taranov, V. V.

AU - de Boer, A. H.

AU - Babakov, A. V.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - A comparative study was performed to analyze the effect of cold acclimation on improving the resistance of Arabidopsis thaliana, Eutrema salsugineum and Eutrema botschantzevii plants to salt stress. Shoot FW, sodium and potassium accumulation, metabolite content, expression of proton pump genes VAB1, VAB2,VAB3, VP2, HA3 and genes encoding ion transporters SOS1, HKT1, NHX1, NHX2, NHX5 located in the plasma membrane or tonoplast were determined just after the cold treatment and the onset of the salt stress. In the same cold-acclimated E. botschantzevii plants, the Na+ concentration after salt treatment was around 80% lower than in non-acclimated plants, whereas the K+ concentration was higher. As a result of cold acclimation, the expression of, VAB3, NHX2, NHX5 genes and of SOS1, VP2, HA3 genes was strongly enhanced in E. botschantzevii and in E. salsugineum plants correspondently. None of the 10 genes analyzed showed any expression change in A. thaliana plants after cold acclimation. Altogether, the results indicate that cold-induced adaptation to subsequent salt stress exists in the extremophytes E. botschantzevii and to a lesser extend in E. salsugineum and is absent in Arabidopsis. This phenomenon may be attributed to the increased expression of ion transporter genes during cold acclimation in the Eutrema species.

AB - A comparative study was performed to analyze the effect of cold acclimation on improving the resistance of Arabidopsis thaliana, Eutrema salsugineum and Eutrema botschantzevii plants to salt stress. Shoot FW, sodium and potassium accumulation, metabolite content, expression of proton pump genes VAB1, VAB2,VAB3, VP2, HA3 and genes encoding ion transporters SOS1, HKT1, NHX1, NHX2, NHX5 located in the plasma membrane or tonoplast were determined just after the cold treatment and the onset of the salt stress. In the same cold-acclimated E. botschantzevii plants, the Na+ concentration after salt treatment was around 80% lower than in non-acclimated plants, whereas the K+ concentration was higher. As a result of cold acclimation, the expression of, VAB3, NHX2, NHX5 genes and of SOS1, VP2, HA3 genes was strongly enhanced in E. botschantzevii and in E. salsugineum plants correspondently. None of the 10 genes analyzed showed any expression change in A. thaliana plants after cold acclimation. Altogether, the results indicate that cold-induced adaptation to subsequent salt stress exists in the extremophytes E. botschantzevii and to a lesser extend in E. salsugineum and is absent in Arabidopsis. This phenomenon may be attributed to the increased expression of ion transporter genes during cold acclimation in the Eutrema species.

KW - Arabidopsis thaliana

KW - Cis-acting elements

KW - Cold acclimation

KW - Eutrema botschantzevii

KW - Eutrema salsugineum

KW - Gene expression

KW - Ion transporter

KW - K/Na accumulation

KW - Metabolite content

KW - Salt stress

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U2 - 10.1016/j.jplph.2016.10.009

DO - 10.1016/j.jplph.2016.10.009

M3 - Article

C2 - 27940414

SN - 0176-1617

VL - 208

SP - 128

EP - 138

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

IS - January

ER -

Cold stress increases salt tolerance of the extremophytes Eutrema salsugineum (Thellungiella salsuginea) and Eutrema (Thellungiella) botschantzevii

Abstract

Funding

UN SDGs

Keywords

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