Comparison of composition and anticaries effect of Galla chinensis extracts with different isolation methods

X. Huang; M. Deng; M. Liu; L. Cheng; R.A.M. Exterkate; J. Li; X. Zhou; J.M. Ten Cate

doi:10.2174/1874210601711010447

Comparison of composition and anticaries effect of Galla chinensis extracts with different isolation methods

X. Huang, M. Deng, M. Liu, L. Cheng, R.A.M. Exterkate, J. Li, X. Zhou, J.M. Ten Cate

Preventive Dentistry

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Objectives: Galla chinensis water extract (GCE) has been demonstrated to inhibit dental caries by favorably shifting the demineralization/remineralization balance of enamel and inhibiting the biomass and acid formation of dental biofilm. The present study focused on the comparison of composition and anticaries effect of Galla chinensis extracts with different isolation methods, aiming to improve the efficacy of caries prevention. Methods: The composition of water extract (GCE), ethanol extract (eGCE) and commercial tannic acid was compared. High performance liquid chromatography coupled to electrospray ionization-time of flight-mass spectrometry (HPLC-ESI-TOF-MS) analysis was used to analyze the main ingredients. In vitro pH-cycling regime and polymicrobial biofilms model were used to assess the ability of different Galla chinensis extracts to inhibit enamel demineralization, acid formation and biofilm formation. Results: All the GCE, eGCE and tannic acid contained a high level of total phenolics. HPLC-ESI-TOF-MS analysis showed that the main ingredients of GCE were gallic acid (GA), while eGCE mainly contained 4-7 galloylglucopyranoses (GGs) and tannic acid mainly contained 5-10 GGs. Furthermore, eGCE and tannic acid showed a better effect on inhibiting enamel demineralization, acid formation and biofilm formation compared to GCE. Conclusions: Galla chinensis extracts with higher tannin content were suggested to have higher potential to prevent dental caries.

Original language	English
Pages (from-to)	447-459
Number of pages	13
Journal	Open Dentistry Journal
Volume	11
DOIs	https://doi.org/10.2174/1874210601711010447 https://doi.org/10.2174/1874210601711010447
Publication status	Published - 2017

Funding

The authors are grateful to the West China School of Pharmacy, Sichuan University for providing technical assistance in Galla chinensis extractions. This study was supported by grants from the Academic Center for Dentistry Amsterdam, the Netherlands and State Key Laboratory of Oral Diseases, Chengdu, China, and National Natural Science Foundation of China grant 81372889.

Funders	Funder number
Academic Center for Dentistry Amsterdam
Netherlands and State Key Laboratory of Oral Diseases, Chengdu, China
National Natural Science Foundation of China	81372889

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{6955b641d836405193338c2cc8224323,

title = "Comparison of composition and anticaries effect of Galla chinensis extracts with different isolation methods",

abstract = "Objectives: Galla chinensis water extract (GCE) has been demonstrated to inhibit dental caries by favorably shifting the demineralization/remineralization balance of enamel and inhibiting the biomass and acid formation of dental biofilm. The present study focused on the comparison of composition and anticaries effect of Galla chinensis extracts with different isolation methods, aiming to improve the efficacy of caries prevention. Methods: The composition of water extract (GCE), ethanol extract (eGCE) and commercial tannic acid was compared. High performance liquid chromatography coupled to electrospray ionization-time of flight-mass spectrometry (HPLC-ESI-TOF-MS) analysis was used to analyze the main ingredients. In vitro pH-cycling regime and polymicrobial biofilms model were used to assess the ability of different Galla chinensis extracts to inhibit enamel demineralization, acid formation and biofilm formation. Results: All the GCE, eGCE and tannic acid contained a high level of total phenolics. HPLC-ESI-TOF-MS analysis showed that the main ingredients of GCE were gallic acid (GA), while eGCE mainly contained 4-7 galloylglucopyranoses (GGs) and tannic acid mainly contained 5-10 GGs. Furthermore, eGCE and tannic acid showed a better effect on inhibiting enamel demineralization, acid formation and biofilm formation compared to GCE. Conclusions: Galla chinensis extracts with higher tannin content were suggested to have higher potential to prevent dental caries.",

author = "X. Huang and M. Deng and M. Liu and L. Cheng and R.A.M. Exterkate and J. Li and X. Zhou and {Ten Cate}, J.M.",

year = "2017",

doi = "10.2174/1874210601711010447",

language = "English",

volume = "11",

pages = "447--459",

journal = "Open Dentistry Journal",

issn = "1874-2106",

publisher = "Bentham Science Publishers",

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T1 - Comparison of composition and anticaries effect of Galla chinensis extracts with different isolation methods

AU - Huang, X.

AU - Deng, M.

AU - Liu, M.

AU - Cheng, L.

AU - Exterkate, R.A.M.

AU - Li, J.

AU - Zhou, X.

AU - Ten Cate, J.M.

PY - 2017

Y1 - 2017

N2 - Objectives: Galla chinensis water extract (GCE) has been demonstrated to inhibit dental caries by favorably shifting the demineralization/remineralization balance of enamel and inhibiting the biomass and acid formation of dental biofilm. The present study focused on the comparison of composition and anticaries effect of Galla chinensis extracts with different isolation methods, aiming to improve the efficacy of caries prevention. Methods: The composition of water extract (GCE), ethanol extract (eGCE) and commercial tannic acid was compared. High performance liquid chromatography coupled to electrospray ionization-time of flight-mass spectrometry (HPLC-ESI-TOF-MS) analysis was used to analyze the main ingredients. In vitro pH-cycling regime and polymicrobial biofilms model were used to assess the ability of different Galla chinensis extracts to inhibit enamel demineralization, acid formation and biofilm formation. Results: All the GCE, eGCE and tannic acid contained a high level of total phenolics. HPLC-ESI-TOF-MS analysis showed that the main ingredients of GCE were gallic acid (GA), while eGCE mainly contained 4-7 galloylglucopyranoses (GGs) and tannic acid mainly contained 5-10 GGs. Furthermore, eGCE and tannic acid showed a better effect on inhibiting enamel demineralization, acid formation and biofilm formation compared to GCE. Conclusions: Galla chinensis extracts with higher tannin content were suggested to have higher potential to prevent dental caries.

AB - Objectives: Galla chinensis water extract (GCE) has been demonstrated to inhibit dental caries by favorably shifting the demineralization/remineralization balance of enamel and inhibiting the biomass and acid formation of dental biofilm. The present study focused on the comparison of composition and anticaries effect of Galla chinensis extracts with different isolation methods, aiming to improve the efficacy of caries prevention. Methods: The composition of water extract (GCE), ethanol extract (eGCE) and commercial tannic acid was compared. High performance liquid chromatography coupled to electrospray ionization-time of flight-mass spectrometry (HPLC-ESI-TOF-MS) analysis was used to analyze the main ingredients. In vitro pH-cycling regime and polymicrobial biofilms model were used to assess the ability of different Galla chinensis extracts to inhibit enamel demineralization, acid formation and biofilm formation. Results: All the GCE, eGCE and tannic acid contained a high level of total phenolics. HPLC-ESI-TOF-MS analysis showed that the main ingredients of GCE were gallic acid (GA), while eGCE mainly contained 4-7 galloylglucopyranoses (GGs) and tannic acid mainly contained 5-10 GGs. Furthermore, eGCE and tannic acid showed a better effect on inhibiting enamel demineralization, acid formation and biofilm formation compared to GCE. Conclusions: Galla chinensis extracts with higher tannin content were suggested to have higher potential to prevent dental caries.

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Comparison of composition and anticaries effect of Galla chinensis extracts with different isolation methods

Abstract

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