Comparison of different liquid chromatography methods for the determination of corticosteroids in biological matrices

A.A.M. Stolker; P.L.W.J. Schwillens; L.A. van Ginkel; U.A.T. Brinkman

doi:10.1016/S0021-9673(00)00725-1

Comparison of different liquid chromatography methods for the determination of corticosteroids in biological matrices

A.A.M. Stolker
, P.L.W.J. Schwillens
, L.A. van Ginkel
, U.A.T. Brinkman

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

Various extraction techniques can be combined with column liquid chromatography (LC) and ultraviolet (UV) or mass spectrometric (MS) detection for the determination of synthetic corticosteroids in biological matrices. Target analysis of low concentrations of 25 μg/kg of dexamethasone in feed can be performed by combining immunoaffinity chromatography (IAC) and LC with UV detection. A straightforward multi-analyte procedure is obtained by tandem solid-phase extraction (SPE) and subsequent LC-UV. However, the limits of detection for feed samples are then relatively poor, viz. 100 μg/kg. A multi-analyte method which meets modern demands of about 5 μg/kg detection limit requires one-step SPE combined with LC-MS analysis. As regards urine corticosteroids can be determined down to a level of 0.5 μg/l by either SPE-LC-MS-MS or SPE(IAC)-LC-MS. Copyright (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	55-67
Number of pages	13
Journal	Journal of Chromatography A
Volume	893
Issue number	1
DOIs	https://doi.org/10.1016/S0021-9673(00)00725-1
Publication status	Published - 2000

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title = "Comparison of different liquid chromatography methods for the determination of corticosteroids in biological matrices",

abstract = "Various extraction techniques can be combined with column liquid chromatography (LC) and ultraviolet (UV) or mass spectrometric (MS) detection for the determination of synthetic corticosteroids in biological matrices. Target analysis of low concentrations of 25 μg/kg of dexamethasone in feed can be performed by combining immunoaffinity chromatography (IAC) and LC with UV detection. A straightforward multi-analyte procedure is obtained by tandem solid-phase extraction (SPE) and subsequent LC-UV. However, the limits of detection for feed samples are then relatively poor, viz. 100 μg/kg. A multi-analyte method which meets modern demands of about 5 μg/kg detection limit requires one-step SPE combined with LC-MS analysis. As regards urine corticosteroids can be determined down to a level of 0.5 μg/l by either SPE-LC-MS-MS or SPE(IAC)-LC-MS. Copyright (C) 2000 Elsevier Science B.V.",

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AU - Stolker, A.A.M.

AU - Schwillens, P.L.W.J.

AU - van Ginkel, L.A.

AU - Brinkman, U.A.T.

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Y1 - 2000

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AB - Various extraction techniques can be combined with column liquid chromatography (LC) and ultraviolet (UV) or mass spectrometric (MS) detection for the determination of synthetic corticosteroids in biological matrices. Target analysis of low concentrations of 25 μg/kg of dexamethasone in feed can be performed by combining immunoaffinity chromatography (IAC) and LC with UV detection. A straightforward multi-analyte procedure is obtained by tandem solid-phase extraction (SPE) and subsequent LC-UV. However, the limits of detection for feed samples are then relatively poor, viz. 100 μg/kg. A multi-analyte method which meets modern demands of about 5 μg/kg detection limit requires one-step SPE combined with LC-MS analysis. As regards urine corticosteroids can be determined down to a level of 0.5 μg/l by either SPE-LC-MS-MS or SPE(IAC)-LC-MS. Copyright (C) 2000 Elsevier Science B.V.

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M3 - Article

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JO - Journal of Chromatography A

JF - Journal of Chromatography A

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ER -

Comparison of different liquid chromatography methods for the determination of corticosteroids in biological matrices

Abstract

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