Comparison of in vitro and in vivo bioassays to measure thyroid hormone disrupting activity in water extracts

Frederic D.L. Leusch, Natalie H. Aneck-Hahn, Jo Anne E. Cavanagh, David Du Pasquier, Timo Hamers, Armelle Hebert, Peta A. Neale, Marco Scheurer, Steven O. Simmons, Merijn Schriks

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Environmental chemicals can induce thyroid disruption through a number of mechanisms including altered thyroid hormone biosynthesis and transport, as well as activation and inhibition of the thyroid receptor. In the current study six in vitro bioassays indicative of different mechanisms of thyroid disruption and one whole animal in vivo assay were applied to 9 model compounds and 4 different water samples (treated wastewater, surface water, drinking water and ultra-pure lab water; both unspiked and spiked with model compounds) to determine their ability to detect thyroid active compounds. Most assays correctly identified and quantified the model compounds as agonists or antagonists, with the reporter gene assays being the most sensitive. However, the reporter gene assays did not detect significant thyroid activity in any of the water samples, suggesting that activation or inhibition of the thyroid hormone receptor is not a relevant mode of action for thyroid endocrine disruptors in water. The thyroperoxidase (TPO) inhibition assay and transthyretin (TTR) displacement assay (FITC) detected activity in the surface water and treated wastewater samples, but more work is required to assess if this activity is a true measure of thyroid activity or matrix interference. The whole animal Xenopus Embryonic Thyroid Assay (XETA) detected some activity in the unspiked surface water and treated wastewater extracts, but not in unspiked drinking water, and appears to be a suitable assay to detect thyroid activity in environmental waters.

LanguageEnglish
Pages868-875
Number of pages8
JournalChemosphere
Volume191
DOIs
StatePublished - 21 Oct 2017

Fingerprint

Bioassay
Thyroid Hormones
hormone
bioassay
Assays
assay
Water
Surface waters
water
Wastewater
surface water
wastewater
Drinking Water
drinking water
Animals
Genes
Chemical activation
endocrine disruptor
gene
animal

Keywords

  • In vitro
  • In vivo
  • Surface water
  • Thyroid activity
  • Wastewater

Cite this

Leusch, F. D. L., Aneck-Hahn, N. H., Cavanagh, J. A. E., Du Pasquier, D., Hamers, T., Hebert, A., ... Schriks, M. (2017). Comparison of in vitro and in vivo bioassays to measure thyroid hormone disrupting activity in water extracts. Chemosphere, 191, 868-875. DOI: 10.1016/j.chemosphere.2017.10.109
Leusch, Frederic D.L. ; Aneck-Hahn, Natalie H. ; Cavanagh, Jo Anne E. ; Du Pasquier, David ; Hamers, Timo ; Hebert, Armelle ; Neale, Peta A. ; Scheurer, Marco ; Simmons, Steven O. ; Schriks, Merijn. / Comparison of in vitro and in vivo bioassays to measure thyroid hormone disrupting activity in water extracts. In: Chemosphere. 2017 ; Vol. 191. pp. 868-875
@article{d8f0c4e4db0b43ee89a4a451fbf52e4f,
title = "Comparison of in vitro and in vivo bioassays to measure thyroid hormone disrupting activity in water extracts",
abstract = "Environmental chemicals can induce thyroid disruption through a number of mechanisms including altered thyroid hormone biosynthesis and transport, as well as activation and inhibition of the thyroid receptor. In the current study six in vitro bioassays indicative of different mechanisms of thyroid disruption and one whole animal in vivo assay were applied to 9 model compounds and 4 different water samples (treated wastewater, surface water, drinking water and ultra-pure lab water; both unspiked and spiked with model compounds) to determine their ability to detect thyroid active compounds. Most assays correctly identified and quantified the model compounds as agonists or antagonists, with the reporter gene assays being the most sensitive. However, the reporter gene assays did not detect significant thyroid activity in any of the water samples, suggesting that activation or inhibition of the thyroid hormone receptor is not a relevant mode of action for thyroid endocrine disruptors in water. The thyroperoxidase (TPO) inhibition assay and transthyretin (TTR) displacement assay (FITC) detected activity in the surface water and treated wastewater samples, but more work is required to assess if this activity is a true measure of thyroid activity or matrix interference. The whole animal Xenopus Embryonic Thyroid Assay (XETA) detected some activity in the unspiked surface water and treated wastewater extracts, but not in unspiked drinking water, and appears to be a suitable assay to detect thyroid activity in environmental waters.",
keywords = "In vitro, In vivo, Surface water, Thyroid activity, Wastewater",
author = "Leusch, {Frederic D.L.} and Aneck-Hahn, {Natalie H.} and Cavanagh, {Jo Anne E.} and {Du Pasquier}, David and Timo Hamers and Armelle Hebert and Neale, {Peta A.} and Marco Scheurer and Simmons, {Steven O.} and Merijn Schriks",
year = "2017",
month = "10",
day = "21",
doi = "10.1016/j.chemosphere.2017.10.109",
language = "English",
volume = "191",
pages = "868--875",
journal = "Chemosphere",
issn = "0045-6535",
publisher = "Elsevier Limited",

}

Leusch, FDL, Aneck-Hahn, NH, Cavanagh, JAE, Du Pasquier, D, Hamers, T, Hebert, A, Neale, PA, Scheurer, M, Simmons, SO & Schriks, M 2017, 'Comparison of in vitro and in vivo bioassays to measure thyroid hormone disrupting activity in water extracts' Chemosphere, vol. 191, pp. 868-875. DOI: 10.1016/j.chemosphere.2017.10.109

Comparison of in vitro and in vivo bioassays to measure thyroid hormone disrupting activity in water extracts. / Leusch, Frederic D.L.; Aneck-Hahn, Natalie H.; Cavanagh, Jo Anne E.; Du Pasquier, David; Hamers, Timo; Hebert, Armelle; Neale, Peta A.; Scheurer, Marco; Simmons, Steven O.; Schriks, Merijn.

In: Chemosphere, Vol. 191, 21.10.2017, p. 868-875.

Research output: Contribution to JournalArticleAcademicpeer-review

TY - JOUR

T1 - Comparison of in vitro and in vivo bioassays to measure thyroid hormone disrupting activity in water extracts

AU - Leusch,Frederic D.L.

AU - Aneck-Hahn,Natalie H.

AU - Cavanagh,Jo Anne E.

AU - Du Pasquier,David

AU - Hamers,Timo

AU - Hebert,Armelle

AU - Neale,Peta A.

AU - Scheurer,Marco

AU - Simmons,Steven O.

AU - Schriks,Merijn

PY - 2017/10/21

Y1 - 2017/10/21

N2 - Environmental chemicals can induce thyroid disruption through a number of mechanisms including altered thyroid hormone biosynthesis and transport, as well as activation and inhibition of the thyroid receptor. In the current study six in vitro bioassays indicative of different mechanisms of thyroid disruption and one whole animal in vivo assay were applied to 9 model compounds and 4 different water samples (treated wastewater, surface water, drinking water and ultra-pure lab water; both unspiked and spiked with model compounds) to determine their ability to detect thyroid active compounds. Most assays correctly identified and quantified the model compounds as agonists or antagonists, with the reporter gene assays being the most sensitive. However, the reporter gene assays did not detect significant thyroid activity in any of the water samples, suggesting that activation or inhibition of the thyroid hormone receptor is not a relevant mode of action for thyroid endocrine disruptors in water. The thyroperoxidase (TPO) inhibition assay and transthyretin (TTR) displacement assay (FITC) detected activity in the surface water and treated wastewater samples, but more work is required to assess if this activity is a true measure of thyroid activity or matrix interference. The whole animal Xenopus Embryonic Thyroid Assay (XETA) detected some activity in the unspiked surface water and treated wastewater extracts, but not in unspiked drinking water, and appears to be a suitable assay to detect thyroid activity in environmental waters.

AB - Environmental chemicals can induce thyroid disruption through a number of mechanisms including altered thyroid hormone biosynthesis and transport, as well as activation and inhibition of the thyroid receptor. In the current study six in vitro bioassays indicative of different mechanisms of thyroid disruption and one whole animal in vivo assay were applied to 9 model compounds and 4 different water samples (treated wastewater, surface water, drinking water and ultra-pure lab water; both unspiked and spiked with model compounds) to determine their ability to detect thyroid active compounds. Most assays correctly identified and quantified the model compounds as agonists or antagonists, with the reporter gene assays being the most sensitive. However, the reporter gene assays did not detect significant thyroid activity in any of the water samples, suggesting that activation or inhibition of the thyroid hormone receptor is not a relevant mode of action for thyroid endocrine disruptors in water. The thyroperoxidase (TPO) inhibition assay and transthyretin (TTR) displacement assay (FITC) detected activity in the surface water and treated wastewater samples, but more work is required to assess if this activity is a true measure of thyroid activity or matrix interference. The whole animal Xenopus Embryonic Thyroid Assay (XETA) detected some activity in the unspiked surface water and treated wastewater extracts, but not in unspiked drinking water, and appears to be a suitable assay to detect thyroid activity in environmental waters.

KW - In vitro

KW - In vivo

KW - Surface water

KW - Thyroid activity

KW - Wastewater

UR - http://www.scopus.com/inward/record.url?scp=85032265689&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032265689&partnerID=8YFLogxK

U2 - 10.1016/j.chemosphere.2017.10.109

DO - 10.1016/j.chemosphere.2017.10.109

M3 - Article

VL - 191

SP - 868

EP - 875

JO - Chemosphere

T2 - Chemosphere

JF - Chemosphere

SN - 0045-6535

ER -

Leusch FDL, Aneck-Hahn NH, Cavanagh JAE, Du Pasquier D, Hamers T, Hebert A et al. Comparison of in vitro and in vivo bioassays to measure thyroid hormone disrupting activity in water extracts. Chemosphere. 2017 Oct 21;191:868-875. Available from, DOI: 10.1016/j.chemosphere.2017.10.109