Comparison of in vitro and in vivo screening models for androgenic and estrogenic activities

E. Sonneveld, H.J. Jansen, S. Man, A.J.G. Jonas, A. Brouwer, B. van der Burg

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Abstract

Identification of nuclear receptor-mediated endocrine activities is important in a variety of fields, ranging from pharmacological and clinical screening, to food and feed safety, toxicological monitoring, and risk assessment. Traditionally animal studies such as the Hershberger and Allen-Doisy tests are used for the assessment of androgenic and estrogenic potencies, respectively. To allow fast analysis of the activities of new chemicals, food additives, and pharmaceutical compounds, high-throughput screening strategies have been developed. Here, a panel of mainly steroidal compounds, screened in different in vitro assays, was compared with two human U2-OS cell line-based CALUX® (Chemically Activated LUciferase eXpression) reporter gene assays for androgens (AR CALUX) and estrogens (ER CALUX). Correlations found between the data of these two CALUX reporter gene assays and data obtained with other in vitro screening assays measuring receptor binding or reporter gene activation (CHO cell line-based) were good (correlation coefficients (r
Original languageEnglish
Pages (from-to)173-187
Number of pages14
JournalToxicological Sciences
Volume89
DOIs
Publication statusPublished - 2006

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