Complementation of Escherichia coli uncD mutant strains by a chimeric F1-beta subunit constructed from E. coli and spinach chloroplast F1-beta

Andreas Burkovski, H Lill, Siegfried Engelbrecht

Research output: Contribution to JournalArticleAcademicpeer-review


ATP-synthesizing F0F1-ATPases are complex enzymes consisting of at least eight different subunits. These subunits are conserved during evolution to a very variable degree ranging in pairwise comparison between, for example, Escherichia coli and spinach chloroplast from 20% to 66% identical residues. It was surprising to find that some of the less well conserved subunits like delta and epsilon could replace their E. coli counterparts, whereas the highly conserved beta subunit, which carries the active site, in the E. coli enzyme could not be substituted by spinach chloroplast beta (Lill et al. (1993) Biochim. Biophys. Acta 1144, 278-284). We constructed a chimeric F1-beta subunit consisting of spinach beta in which the 96 N-terminal amino acids were replaced by the respective residue sequence from E. coli beta. Whereas spinach beta did not complement E. coli uncD mutant strains, the chimeric beta subunit restored growth under conditions of oxidative phosphorylation.

Original languageEnglish
Pages (from-to)243-6
Number of pages4
JournalBiochimica et Biophysica Acta
Issue number3
Publication statusPublished - 29 Jul 1994



  • Amino Acid Sequence
  • Chimera
  • Chloroplasts
  • Escherichia coli
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Plants, Edible
  • Proton-Translocating ATPases
  • Sequence Alignment
  • Journal Article
  • Research Support, Non-U.S. Gov't

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