Composition of the type VII secretion system membrane complex.

E.N.G. Houben, J. Bestebroer, R. Ummels, L. Wilson, S.R. Piersma, C.R. Jimenez, T.H.M. Ottenhoff, S. Luirink, W. Bitter

Research output: Contribution to JournalArticleAcademicpeer-review


Pathogenic mycobacteria require type VII secretion (T7S) systems to transport virulence factors across their complex cell envelope. These bacteria have up to five of these systems, termed ESX-1 to ESX-5. Here, we show that ESX-5 of Mycobacterium tuberculosis mediates the secretion of EsxN, PPE and PE_PGRS proteins, indicating that ESX-5 is a major secretion pathway in this important pathogen. Using the ESX-5 system of Mycobacterium marinum and Mycobacterium bovis BCG as a model, we have purified and analysed the T7S membrane complex under native conditions. blue native-PAGE and immunoprecipitation experiments showed that the ESX-5 membrane complex of both species has a size of ~1500kDa and is composed of four conserved membrane proteins, i.e. EccB
Original languageEnglish
Pages (from-to)472-484
JournalMolecular Microbiology
Issue number2
Early online date27 Aug 2012
Publication statusPublished - 2012


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