Composition of the type VII secretion system membrane complex.

E.N.G. Houben, J. Bestebroer, R. Ummels, L. Wilson, S.R. Piersma, C.R. Jimenez, T.H.M. Ottenhoff, S. Luirink, W. Bitter

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Pathogenic mycobacteria require type VII secretion (T7S) systems to transport virulence factors across their complex cell envelope. These bacteria have up to five of these systems, termed ESX-1 to ESX-5. Here, we show that ESX-5 of Mycobacterium tuberculosis mediates the secretion of EsxN, PPE and PE_PGRS proteins, indicating that ESX-5 is a major secretion pathway in this important pathogen. Using the ESX-5 system of Mycobacterium marinum and Mycobacterium bovis BCG as a model, we have purified and analysed the T7S membrane complex under native conditions. blue native-PAGE and immunoprecipitation experiments showed that the ESX-5 membrane complex of both species has a size of ~1500kDa and is composed of four conserved membrane proteins, i.e. EccB
Original languageEnglish
Pages (from-to)472-484
JournalMolecular Microbiology
Volume86
Issue number2
Early online date27 Aug 2012
DOIs
Publication statusPublished - 2012

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Mycobacterium bovis
Mycobacterium marinum
Native Polyacrylamide Gel Electrophoresis
Membranes
Secretory Pathway
Virulence Factors
Mycobacterium
Mycobacterium tuberculosis
Immunoprecipitation
Membrane Proteins
Bacteria
Proteins
Type VII Secretion Systems

Cite this

Houben, E.N.G. ; Bestebroer, J. ; Ummels, R. ; Wilson, L. ; Piersma, S.R. ; Jimenez, C.R. ; Ottenhoff, T.H.M. ; Luirink, S. ; Bitter, W. / Composition of the type VII secretion system membrane complex. In: Molecular Microbiology. 2012 ; Vol. 86, No. 2. pp. 472-484.
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author = "E.N.G. Houben and J. Bestebroer and R. Ummels and L. Wilson and S.R. Piersma and C.R. Jimenez and T.H.M. Ottenhoff and S. Luirink and W. Bitter",
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Houben, ENG, Bestebroer, J, Ummels, R, Wilson, L, Piersma, SR, Jimenez, CR, Ottenhoff, THM, Luirink, S & Bitter, W 2012, 'Composition of the type VII secretion system membrane complex.' Molecular Microbiology, vol. 86, no. 2, pp. 472-484. https://doi.org/10.1111/j.1365-2958.2012.08206.x

Composition of the type VII secretion system membrane complex. / Houben, E.N.G.; Bestebroer, J.; Ummels, R.; Wilson, L.; Piersma, S.R.; Jimenez, C.R.; Ottenhoff, T.H.M.; Luirink, S.; Bitter, W.

In: Molecular Microbiology, Vol. 86, No. 2, 2012, p. 472-484.

Research output: Contribution to JournalArticleAcademicpeer-review

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AU - Bitter, W.

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