Comprehensive Morpho-Electrotonic Analysis Shows 2 Distinct Classes of L2 and L3 Pyramidal Neurons in Human Temporal Cortex

Yair Deitcher, Guy Eyal, Lida Kanari, Matthijs B Verhoog, Guy Antoine Atenekeng Kahou, Huibert D Mansvelder, Christiaan P J de Kock, Idan Segev

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

There have been few quantitative characterizations of the morphological, biophysical, and cable properties of neurons in the human neocortex. We employed feature-based statistical methods on a rare data set of 60 3D reconstructed pyramidal neurons from L2 and L3 in the human temporal cortex (HL2/L3 PCs) removed after brain surgery. Of these cells, 25 neurons were also characterized physiologically. Thirty-two morphological features were analyzed (e.g., dendritic surface area, 36 333 ± 18 157 μm2; number of basal trees, 5.55 ± 1.47; dendritic diameter, 0.76 ± 0.28 μm). Eighteen features showed a significant gradual increase with depth from the pia (e.g., dendritic length and soma radius). The other features showed weak or no correlation with depth (e.g., dendritic diameter). The basal dendritic terminals in HL2/L3 PCs are particularly elongated, enabling multiple nonlinear processing units in these dendrites. Unlike the morphological features, the active biophysical features (e.g., spike shapes and rates) and passive/cable features (e.g., somatic input resistance, 47.68 ± 15.26 MΩ, membrane time constant, 12.03 ± 1.79 ms, average dendritic cable length, 0.99 ± 0.24) were depth-independent. A novel descriptor for apical dendritic topology yielded 2 distinct classes, termed hereby as "slim-tufted" and "profuse-tufted" HL2/L3 PCs; the latter class tends to fire at higher rates. Thus, our morpho-electrotonic analysis shows 2 distinct classes of HL2/L3 PCs.

Original languageEnglish
Pages (from-to)5398-5414
Number of pages17
JournalCerebral Cortex
Volume27
Issue number11
DOIs
Publication statusPublished - 1 Nov 2017

Funding

I.S. was supported by grant agreement no. 604102 “Human Brain Project” and by a grant from the Gatsby Charitable Foundation. H. D.M. received funding for this work from the Netherlands Organization for Scientific Research (NWO; VICI grant), ERC StG “BrainSignals”, and EU H2020 grant agreement no. 604102 “Human Brain Project”. Part of this project was supported by Hersenstichting Nederland (grant HSN 2010(1)-09) to C.P.J.D.K.

FundersFunder number
EU H2020
Netherlands Organization for Scientific Research
Seventh Framework Programme242167, 604102
Gatsby Charitable Foundation
European Research Council
Nederlandse Organisatie voor Wetenschappelijk Onderzoek
HersenstichtingHSN 2010(1)-09

    Keywords

    • Journal Article

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