Confocal corrected attenuation coefficient imaging in phantoms and in vivo using chromatic focal shift calibration

Johannes Kübler, Vincent S. Zoutenbier, Gijs Buist, Jörg Fischer, Arjen Amelink, Johannes F. de Boer

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Optical coherence tomography (OCT) is conventionally used for structural imaging of tissue. Calibrating the intensity values of OCT images can give information on the tissue’s inherent optical properties, such as the attenuation coefficient, which can provide an additional parameter to quantify possible pathological changes. To obtain calibrated intensity values, the focus position and Rayleigh length of the incident beam need to be known. We explore the feasibility of extracting the focus position from an OCT scan acquired with a single focus setting using the chromatic aberration of the system. The chromatic focal shift of an OCT system is exploited to achieve different focus positions for sub-spectrum reconstructed OCT images. The ratios of these images are used to estimate the focus position. Reconstruction of a high-resolution B-scan from coherent addition of sub-spectrum confocal function corrected B-scans and subsequent high-resolution OCT attenuation coefficient imaging is demonstrated. Furthermore, we introduce a method to experimentally determine the chromatic focal shifts of an OCT system in phantoms and an in vivo human retina. These shifts are compared to the theoretically expected shifts calculated with ray tracing.
Original languageEnglish
Pages (from-to)5282-5297
JournalBiomedical Optics Express
Volume14
Issue number10
DOIs
Publication statusPublished - 1 Oct 2023

Funding

Heidelberg Engineering GmbH; Health∼Holland, Topsector Life Sciences & Health; Holland High Tech, Topsector High Tech Systems and Materials.

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