Conformational switch of syntaxin-1 controls synaptic vesicle fusion

S.H. Gerber; J.C. Rah; X. Liu; H. de Wit; I. Dulubova; A.C. Meyer; J. Rizo; M. Arancillo; R.E. Hammer; M. Verhage; C. Rosenmund; T.C. Südhof

doi:10.1126/science.1163174

Conformational switch of syntaxin-1 controls synaptic vesicle fusion

S.H. Gerber, J.C. Rah, X. Liu, H. de Wit, I. Dulubova, A.C. Meyer, J. Rizo, M. Arancillo, R.E. Hammer, M. Verhage, C. Rosenmund, T.C. Südhof

Functional Genomics

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

During synaptic vesicle fusion, the soluble N-ethylmaleimide-sensitive factor-attachment protein receptor (SNARE) protein syntaxin-1 exhibits two conformations that both bind to Munc18-1: a "closed" conformation outside the SNARE complex and an "open" conformation in the SNARE complex. Although SNARE complexes containing open syntaxin-1 and Munc18-1 are essential for exocytosis, the function of closed syntaxin-1 is unknown. We generated knockin/knockout mice that expressed only open syntaxin-1B. Syntaxin-1B

Original language	English
Pages (from-to)	1507-1510
Journal	Science
Volume	321
DOIs	https://doi.org/10.1126/science.1163174
Publication status	Published - 2008

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10.1126/science.1163174

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title = "Conformational switch of syntaxin-1 controls synaptic vesicle fusion",

abstract = "During synaptic vesicle fusion, the soluble N-ethylmaleimide-sensitive factor-attachment protein receptor (SNARE) protein syntaxin-1 exhibits two conformations that both bind to Munc18-1: a {"}closed{"} conformation outside the SNARE complex and an {"}open{"} conformation in the SNARE complex. Although SNARE complexes containing open syntaxin-1 and Munc18-1 are essential for exocytosis, the function of closed syntaxin-1 is unknown. We generated knockin/knockout mice that expressed only open syntaxin-1B. Syntaxin-1B",

author = "S.H. Gerber and J.C. Rah and X. Liu and {de Wit}, H. and I. Dulubova and A.C. Meyer and J. Rizo and M. Arancillo and R.E. Hammer and M. Verhage and C. Rosenmund and T.C. S{\"u}dhof",

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doi = "10.1126/science.1163174",

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T1 - Conformational switch of syntaxin-1 controls synaptic vesicle fusion

AU - Gerber, S.H.

AU - Rah, J.C.

AU - Liu, X.

AU - de Wit, H.

AU - Dulubova, I.

AU - Meyer, A.C.

AU - Rizo, J.

AU - Arancillo, M.

AU - Hammer, R.E.

AU - Verhage, M.

AU - Rosenmund, C.

AU - Südhof, T.C.

PY - 2008

Y1 - 2008

N2 - During synaptic vesicle fusion, the soluble N-ethylmaleimide-sensitive factor-attachment protein receptor (SNARE) protein syntaxin-1 exhibits two conformations that both bind to Munc18-1: a "closed" conformation outside the SNARE complex and an "open" conformation in the SNARE complex. Although SNARE complexes containing open syntaxin-1 and Munc18-1 are essential for exocytosis, the function of closed syntaxin-1 is unknown. We generated knockin/knockout mice that expressed only open syntaxin-1B. Syntaxin-1B

AB - During synaptic vesicle fusion, the soluble N-ethylmaleimide-sensitive factor-attachment protein receptor (SNARE) protein syntaxin-1 exhibits two conformations that both bind to Munc18-1: a "closed" conformation outside the SNARE complex and an "open" conformation in the SNARE complex. Although SNARE complexes containing open syntaxin-1 and Munc18-1 are essential for exocytosis, the function of closed syntaxin-1 is unknown. We generated knockin/knockout mice that expressed only open syntaxin-1B. Syntaxin-1B

U2 - 10.1126/science.1163174

DO - 10.1126/science.1163174

M3 - Article

SN - 0036-8075

VL - 321

SP - 1507

EP - 1510

JO - Science

JF - Science

ER -

Conformational switch of syntaxin-1 controls synaptic vesicle fusion

Abstract

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