Conformational switch of syntaxin-1 controls synaptic vesicle fusion

S.H. Gerber; J.C. Rah; X. Liu; H. de Wit; I. Dulubova; A.C. Meyer; J. Rizo; M. Arancillo; R.E. Hammer; M. Verhage; C. Rosenmund; T.C. Südhof

doi:10.1126/science.1163174

Conformational switch of syntaxin-1 controls synaptic vesicle fusion

S.H. Gerber
, J.C. Rah
, X. Liu
, H. de Wit
, I. Dulubova
, A.C. Meyer
, J. Rizo
, M. Arancillo
, R.E. Hammer
, M. Verhage
, C. Rosenmund
, T.C. Südhof

Functional Genomics

Research output: Contribution to Journal › Article › Academic › peer-review

Abstract

During synaptic vesicle fusion, the soluble N-ethylmaleimide-sensitive factor-attachment protein receptor (SNARE) protein syntaxin-1 exhibits two conformations that both bind to Munc18-1: a "closed" conformation outside the SNARE complex and an "open" conformation in the SNARE complex. Although SNARE complexes containing open syntaxin-1 and Munc18-1 are essential for exocytosis, the function of closed syntaxin-1 is unknown. We generated knockin/knockout mice that expressed only open syntaxin-1B. Syntaxin-1B

Original language	English
Pages (from-to)	1507-1510
Journal	Science
Volume	321
DOIs	https://doi.org/10.1126/science.1163174
Publication status	Published - 2008

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10.1126/science.1163174

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title = "Conformational switch of syntaxin-1 controls synaptic vesicle fusion",

abstract = "During synaptic vesicle fusion, the soluble N-ethylmaleimide-sensitive factor-attachment protein receptor (SNARE) protein syntaxin-1 exhibits two conformations that both bind to Munc18-1: a {"}closed{"} conformation outside the SNARE complex and an {"}open{"} conformation in the SNARE complex. Although SNARE complexes containing open syntaxin-1 and Munc18-1 are essential for exocytosis, the function of closed syntaxin-1 is unknown. We generated knockin/knockout mice that expressed only open syntaxin-1B. Syntaxin-1B",

author = "S.H. Gerber and J.C. Rah and X. Liu and \{de Wit\}, H. and I. Dulubova and A.C. Meyer and J. Rizo and M. Arancillo and R.E. Hammer and M. Verhage and C. Rosenmund and T.C. S{\"u}dhof",

year = "2008",

doi = "10.1126/science.1163174",

language = "English",

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T1 - Conformational switch of syntaxin-1 controls synaptic vesicle fusion

AU - Gerber, S.H.

AU - Rah, J.C.

AU - Liu, X.

AU - de Wit, H.

AU - Dulubova, I.

AU - Meyer, A.C.

AU - Rizo, J.

AU - Arancillo, M.

AU - Hammer, R.E.

AU - Verhage, M.

AU - Rosenmund, C.

AU - Südhof, T.C.

PY - 2008

Y1 - 2008

N2 - During synaptic vesicle fusion, the soluble N-ethylmaleimide-sensitive factor-attachment protein receptor (SNARE) protein syntaxin-1 exhibits two conformations that both bind to Munc18-1: a "closed" conformation outside the SNARE complex and an "open" conformation in the SNARE complex. Although SNARE complexes containing open syntaxin-1 and Munc18-1 are essential for exocytosis, the function of closed syntaxin-1 is unknown. We generated knockin/knockout mice that expressed only open syntaxin-1B. Syntaxin-1B

AB - During synaptic vesicle fusion, the soluble N-ethylmaleimide-sensitive factor-attachment protein receptor (SNARE) protein syntaxin-1 exhibits two conformations that both bind to Munc18-1: a "closed" conformation outside the SNARE complex and an "open" conformation in the SNARE complex. Although SNARE complexes containing open syntaxin-1 and Munc18-1 are essential for exocytosis, the function of closed syntaxin-1 is unknown. We generated knockin/knockout mice that expressed only open syntaxin-1B. Syntaxin-1B

U2 - 10.1126/science.1163174

DO - 10.1126/science.1163174

M3 - Article

SN - 0036-8075

VL - 321

SP - 1507

EP - 1510

JO - Science

JF - Science

ER -

Conformational switch of syntaxin-1 controls synaptic vesicle fusion

Abstract

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