Consequences of depletion of the signal recognition particle in Escherichia coli

D. Wickstrom, S. Wagner, L Baars, A.J. Ytterberg, M. Klepsch, K.J. van Wijk, J. Luirink, J.W. de Gier

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Thus far, the role of the Escherichia coli signal recognition particle (SRP) has only been studied using targeted approaches. It has been shown for a handful of cytoplasmic membrane proteins that their insertion into the cytoplasmic membrane is at least partially SRP-dependent. Furthermore, it has been proposed that the SRP plays a role in preventing toxic accumulation of mistargeted cytoplasmic membrane proteins in the cytoplasm. To complement the targeted studies on SRP, we have studied the consequences of the depletion of the SRP component Fifty-four homologue (Ffh) in E. coli using a global approach. The steady-state proteomes and the proteome dynamics were evaluated using oneand two-dimensional gel analysis, followed by mass spectrometry-based protein identification and immunoblotting. Our analysis showed that depletion of Ffh led to the following: (i) impaired kinetics of the biogenesis of the cytoplasmic membrane proteome; (ii) lowered steady-state levels of the respiratory complexes NADH dehydrogenase, succinate dehydrogenase, and cytochrome bo
LanguageEnglish
Pages4598-609
Number of pages11
JournalJournal of Biological Chemistry
Volume286
Issue number286
DOIs
Publication statusPublished - 2011

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Signal Recognition Particle
Escherichia coli
Proteome
Cell Membrane
Membrane Proteins
Membranes
NADH Dehydrogenase
Succinate Dehydrogenase
Poisons
Cytochromes
Immunoblotting
Mass spectrometry
Mass Spectrometry
Cytoplasm
Gels
Kinetics
Proteins

Bibliographical note

1083-351x Wickstrom, David Wagner, Samuel Baars, Louise Ytterberg, A Jimmy Klepsch, Mirjam van Wijk, Klaas J Luirink, Joen de Gier, Jan-Willem 5R01GM081827-03/GM/NIGMS NIH HHS/United States Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't United States J Biol Chem. 2011 Feb 11;286(6):4598-609. doi: 10.1074/jbc.M109.081935. Epub 2010 Oct 5.

Cite this

Wickstrom, D., Wagner, S., Baars, L., Ytterberg, A. J., Klepsch, M., van Wijk, K. J., ... de Gier, J. W. (2011). Consequences of depletion of the signal recognition particle in Escherichia coli. Journal of Biological Chemistry, 286(286), 4598-609. https://doi.org/10.1074/jbc.M109.081935
Wickstrom, D. ; Wagner, S. ; Baars, L ; Ytterberg, A.J. ; Klepsch, M. ; van Wijk, K.J. ; Luirink, J. ; de Gier, J.W. / Consequences of depletion of the signal recognition particle in Escherichia coli. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 286. pp. 4598-609.
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Wickstrom, D, Wagner, S, Baars, L, Ytterberg, AJ, Klepsch, M, van Wijk, KJ, Luirink, J & de Gier, JW 2011, 'Consequences of depletion of the signal recognition particle in Escherichia coli', Journal of Biological Chemistry, vol. 286, no. 286, pp. 4598-609. https://doi.org/10.1074/jbc.M109.081935

Consequences of depletion of the signal recognition particle in Escherichia coli. / Wickstrom, D.; Wagner, S.; Baars, L; Ytterberg, A.J.; Klepsch, M.; van Wijk, K.J.; Luirink, J.; de Gier, J.W.

In: Journal of Biological Chemistry, Vol. 286, No. 286, 2011, p. 4598-609.

Research output: Contribution to JournalArticleAcademicpeer-review

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AB - Thus far, the role of the Escherichia coli signal recognition particle (SRP) has only been studied using targeted approaches. It has been shown for a handful of cytoplasmic membrane proteins that their insertion into the cytoplasmic membrane is at least partially SRP-dependent. Furthermore, it has been proposed that the SRP plays a role in preventing toxic accumulation of mistargeted cytoplasmic membrane proteins in the cytoplasm. To complement the targeted studies on SRP, we have studied the consequences of the depletion of the SRP component Fifty-four homologue (Ffh) in E. coli using a global approach. The steady-state proteomes and the proteome dynamics were evaluated using oneand two-dimensional gel analysis, followed by mass spectrometry-based protein identification and immunoblotting. Our analysis showed that depletion of Ffh led to the following: (i) impaired kinetics of the biogenesis of the cytoplasmic membrane proteome; (ii) lowered steady-state levels of the respiratory complexes NADH dehydrogenase, succinate dehydrogenase, and cytochrome bo

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Wickstrom D, Wagner S, Baars L, Ytterberg AJ, Klepsch M, van Wijk KJ et al. Consequences of depletion of the signal recognition particle in Escherichia coli. Journal of Biological Chemistry. 2011;286(286):4598-609. https://doi.org/10.1074/jbc.M109.081935