Contribution of the FtsQ transmembrane segment to loclization to the cell division site.

D.J. Scheffers, C. Robichon, T. den Blaauwen, G.J. Haan, G.M. Koningstein, D.W. van Bloois, J. Beckwith, S. Luirink

Research output: Contribution to JournalArticleAcademic

Abstract

The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Original languageEnglish
Pages (from-to)7273-7280
JournalJournal of Bacteriology
Volume189
Issue number20
DOIs
Publication statusPublished - 2007

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