Contribution of the FtsQ transmembrane segment to loclization to the cell division site.

D.J. Scheffers; C. Robichon; T. den Blaauwen; G.J. Haan; G.M. Koningstein; D.W. van Bloois; J. Beckwith; S. Luirink

doi:10.1128/JB.00723-07

Contribution of the FtsQ transmembrane segment to loclization to the cell division site.

D.J. Scheffers, C. Robichon, T. den Blaauwen, G.J. Haan, G.M. Koningstein, D.W. van Bloois, J. Beckwith, S. Luirink

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Abstract

The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Original language	English
Pages (from-to)	7273-7280
Journal	Journal of Bacteriology
Volume	189
Issue number	20
DOIs	https://doi.org/10.1128/JB.00723-07
Publication status	Published - 2007

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title = "Contribution of the FtsQ transmembrane segment to loclization to the cell division site.",

abstract = "The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site. Copyright {\textcopyright} 2007, American Society for Microbiology. All Rights Reserved.",

author = "D.J. Scheffers and C. Robichon and {den Blaauwen}, T. and G.J. Haan and G.M. Koningstein and {van Bloois}, D.W. and J. Beckwith and S. Luirink",

year = "2007",

doi = "10.1128/JB.00723-07",

language = "English",

volume = "189",

pages = "7273--7280",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Contribution of the FtsQ transmembrane segment to loclization to the cell division site.

AU - Scheffers, D.J.

AU - Robichon, C.

AU - den Blaauwen, T.

AU - Haan, G.J.

AU - Koningstein, G.M.

AU - van Bloois, D.W.

AU - Beckwith, J.

AU - Luirink, S.

PY - 2007

Y1 - 2007

N2 - The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

AB - The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made and analyzed for the ability to complement ftsQ mutant. Study of the various steps involved in FtsQ biogenesis revealed that one mutant (L29/32R;V38P) failed to functionally insert into the membrane, whereas another mutant (L29/32R) was correctly assembled and interacted with FtsB and FtsL but failed to localize efficiently to the cell division site. Our results indicate that the FtsQ TMS plays a role in FtsQ localization to the division site. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

U2 - 10.1128/JB.00723-07

DO - 10.1128/JB.00723-07

M3 - Article

SN - 0021-9193

VL - 189

SP - 7273

EP - 7280

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 20

ER -

Contribution of the FtsQ transmembrane segment to loclization to the cell division site.

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